Rice R L, Schurig G G, Blodgett D J
Department of Biomedical Sciences and Pathobiology, Virginia-Maryland Regional College of Veterinary Medicine, Virginia Tech and University of Maryland, Blacksburg 24061-0442, USA.
Am J Vet Res. 1998 Oct;59(10):1258-62.
To assess effects of vaccination against fescue toxicosis on weight gain, serum prolactin and cholesterol concentrations, and alkaline phosphatase (ALP) activity in mice fed an endophyte-infected (EI) or endophyte-free (EF) fescue diet.
50 six-week-old male BALB/c mice.
Mice were randomly allocated to the following 5 groups: 1, vaccinated intraperitoneally with a bovine serum albumin-ergotamine (EG) conjugate and fed an EI fescue diet; 2, orally vaccinated with cholera toxin (CT) subunit B-EG conjugate mixed with free CT and fed an EI fescue diet; 3, not vaccinated and fed an EI fescue diet; 4, passively vaccinated with monoclonal antibodies specific for ergovaline (EV) and fed an EI fescue diet; and 5, not vaccinated and fed an EF fescue diet.
Antibodies against EG and EV were in serum of mice of groups 1 and 4, respectively. Secretory IgA and IgG coproantibodies against EG were induced in mice of group 2. Weight increased in groups 1 and 2 and tended to be increased in group 4 versus group 3. Prolactin concentration was similar in all groups; cholesterol concentration was decreased in groups 1, 3, and 4, compared with group 5. Compared with that in group 5, serum ALP activity decreased in groups 1 and 4 and was further decreased in group 1, compared with that in groups 2 and 3; it was negatively correlated with anti-EG titer.
Induction of anti-EG antibodies and administration of EV monoclonal antibodies tended to increase short-term weight gain in this murine model of fescue toxicosis. However, systemic IgG antibodies against EG or EV antibodies were not protective against decreases in serum ALP activity and cholesterol concentrations. Clinical significance of decreased ALP activity associated with vaccination is unknown, but represents a worsening of a response often associated with fescue toxicosis in cattle.
评估针对高羊茅中毒的疫苗接种对食用内生菌感染（EI）或无内生菌（EF）高羊茅日粮小鼠体重增加、血清催乳素和胆固醇浓度以及碱性磷酸酶（ALP）活性的影响。
50只六周龄雄性BALB/c小鼠。
将小鼠随机分为以下5组：1组，腹腔注射牛血清白蛋白 - 麦角胺（EG）偶联物并喂食EI高羊茅日粮；2组，口服霍乱毒素（CT）B亚基 - EG偶联物与游离CT混合并喂食EI高羊茅日粮；3组，未接种疫苗并喂食EI高羊茅日粮；4组，被动接种对麦角缬碱（EV）特异的单克隆抗体并喂食EI高羊茅日粮；5组，未接种疫苗并喂食EF高羊茅日粮。
1组和4组小鼠血清中分别出现了针对EG和EV的抗体。2组小鼠诱导产生了针对EG的分泌型IgA和IgG共同抗体。与3组相比，1组和2组体重增加，4组体重有增加趋势。所有组催乳素浓度相似；与5组相比，1组、3组和4组胆固醇浓度降低。与5组相比，1组和4组血清ALP活性降低，与2组和3组相比，1组血清ALP活性进一步降低；其与抗EG效价呈负相关。
在该高羊茅中毒小鼠模型中，诱导产生抗EG抗体和给予EV单克隆抗体倾向于增加短期体重增加。然而，针对EG的全身性IgG抗体或EV抗体并不能保护血清ALP活性和胆固醇浓度降低。与疫苗接种相关的ALP活性降低的临床意义尚不清楚，但代表了牛高羊茅中毒常见反应的恶化。
