Ivanov I P, Gesteland R F, Atkins J F
Department of Human Genetics, University of Utah, Salt Lake City, Utah, 84112, USA.
Genomics. 1998 Sep 1;52(2):119-29. doi: 10.1006/geno.1998.5434.
A second mammalian ornithine decarboxylase antizyme was discovered. The deduced protein sequence of the human antizyme2 is 54% identical and 67% similar to human antizyme1 but 99.5% identical to mouse antizyme2. Polyamine-regulated programmed ribosomal frameshifting is used in decoding antizyme2 mRNA as it is for antizyme1 mRNA. The mRNA signals for the programmed frameshifting are similar in the mRNAs for the two antizymes. However, in the stimulatory pseudoknot 3' of the shift site, while the sequences of the stems are highly conserved, the sequences of the loops are divergent. Functional distinctions between antizymes seem likely, but no distinction in the tissue distribution of human antizyme1 and 2 mRNAs was distinguished, though antizyme2 mRNA is 16-fold less abundant than its antizyme1 counterpart. In addition to the previously characterized human antizyme1 mRNA, a second antizyme1 mRNA with an additional 160 nucleotides at its 3' end was identified, and it has a tissue distribution different from that of the shorter antizyme1 mRNA.
发现了第二种哺乳动物鸟氨酸脱羧酶抗酶。人抗酶2推导的蛋白质序列与人类抗酶1有54%的同一性和67%的相似性，但与小鼠抗酶2有99.5%的同一性。多胺调节的程序性核糖体移码用于解读抗酶2 mRNA，就像解读抗酶1 mRNA一样。两种抗酶mRNA中程序性移码的mRNA信号相似。然而，在移码位点3'端的刺激性假结中，虽然茎的序列高度保守，但环的序列是不同的。抗酶之间似乎存在功能差异，但人抗酶1和抗酶2 mRNA的组织分布没有区别，尽管抗酶2 mRNA的丰度比其对应的抗酶1少16倍。除了先前鉴定的人抗酶1 mRNA外，还鉴定出了另一种在其3'端多了160个核苷酸的抗酶1 mRNA，其组织分布与较短的抗酶1 mRNA不同。
