大鼠脑干中突触前钙电流的易化作用。
Facilitation of presynaptic calcium currents in the rat brainstem.
作者信息
Borst J G, Sakmann B
机构信息
Abteilung Zellphysiologie, Max-Planck-Institut fur medizinische Forschung, Jahnstrasse 29, D-69120 Heidelberg, Germany.
出版信息
J Physiol. 1998 Nov 15;513 ( Pt 1)(Pt 1):149-55. doi: 10.1111/j.1469-7793.1998.149by.x.
Abstract
- To study use-dependent changes in the presynaptic Ca2+ influx and their contribution to transmitter release, we made simultaneous voltage clamp recordings from presynaptic terminals (the calyces of Held) and postsynaptic cells (the principal cells of the medial nucleus of the trapezoid body) in slices of the rat auditory brainstem. 2. Following a short (2 ms) prepulse to 0 mV, calcium channels opened faster during steps to negative test potentials. During trains of action potential waveforms the Ca2+ influx per action potential increased. At the same time, however, the amplitude of the EPSCs decreased. 3. The facilitation of the calcium currents appeared to depend on a build-up of intracellular Ca2+, since its magnitude was proportional to the Ca2+ influx and it was reduced in the presence of 10 mM BAPTA. 4. Facilitation of the presynaptic calcium currents may contribute to short-term facilitation of transmitter release, observed when quantal output is low. Alternatively, it may counteract processes that contribute to synaptic depression.
摘要
- 为了研究突触前钙离子内流的使用依赖性变化及其对递质释放的作用，我们在大鼠听觉脑干切片中，对突触前终末（壶腹）和突触后细胞（梯形体内侧核的主细胞）进行了同步电压钳记录。2. 在向0 mV施加一个短暂（2毫秒）的预脉冲后，在向负性测试电位的阶跃过程中，钙通道开放得更快。在一串动作电位波形期间，每个动作电位的钙离子内流增加。然而，与此同时，兴奋性突触后电流（EPSCs）的幅度减小。3. 钙电流的易化似乎依赖于细胞内钙离子的积累，因为其大小与钙离子内流成正比，并且在存在10 mM 1,2-双（2-氨基苯氧基）乙烷-N,N,N',N'-四乙酸（BAPTA）时会降低。4. 突触前钙电流的易化可能有助于在量子输出较低时观察到的递质释放的短期易化。或者，它可能抵消导致突触抑制 的过程。
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