Ohtsuka N, Yamada Y K, Saeki K, Taguchi F
National Institute of Neuroscience, NCNP, Tokyo, Japan.
Adv Exp Med Biol. 1998;440:77-80. doi: 10.1007/978-1-4615-5331-1_10.
We compared the virus-binding activity and receptor-functionality of the receptor proteins isolated from mouse hepatitis virus (MHV)-susceptible BALB/c mice (MHVR1) and MHV-resistant SJL mice (MHVR2). By using a soluble receptor protein which lacked the transmembrane and intracytoplasmic domains, virus overlay protein blot assay and neutralization tests showed that MHVR1 bound to JHM cl-2 virus with 300-500 times higher efficiency than to MHVR2. MHVR1 was revealed to have 10-30 fold higher receptor-functionality than MHVR2 when examined by measuring virus-binding to the receptor expressed on the cell surface. These findings suggested that the differences in susceptibility between BALB/c and SJL mice may depend upon the genotype of the MHV receptor.
我们比较了从小鼠肝炎病毒（MHV）易感的BALB/c小鼠（MHVR1）和MHV抗性的SJL小鼠（MHVR2）中分离出的受体蛋白的病毒结合活性和受体功能。通过使用缺乏跨膜和胞质内结构域的可溶性受体蛋白，病毒覆盖蛋白印迹分析和中和试验表明，MHVR1与JHM cl-2病毒的结合效率比MHVR2高300-500倍。当通过测量病毒与细胞表面表达的受体的结合来检测时，发现MHVR1的受体功能比MHVR2高10-30倍。这些发现表明，BALB/c和SJL小鼠之间易感性的差异可能取决于MHV受体的基因型。
