人冠状病毒229E 3C样蛋白酶的底物特异性
Substrate specificity of the human coronavirus 229E 3C-like proteinase.
作者信息
Ziebuhr J, Heusipp G, Seybert A, Siddell S G
机构信息
Institute of Virology, University of Würzburg, Germany.
出版信息
Adv Exp Med Biol. 1998;440:115-20. doi: 10.1007/978-1-4615-5331-1_15.
Coronavirus gene expression involves proteolytic processing of the gene 1-encoded polyproteins and a key enzyme in this process is the virus-encoded 3C-like proteinase. In this study, we describe the biosynthesis of the human coronavirus 229E 3C-like proteinase in Escherichia coli and the substrate specificity of the purified protein. Using immunofluorescence microscopy, we have also investigated the subcellular localization of the 3C-like proteinase and have found a punctate, perinuclear distribution of the proteinase in virus-infected cells.
冠状病毒基因表达涉及基因1编码的多聚蛋白的蛋白水解加工，而此过程中的一种关键酶是病毒编码的3C样蛋白酶。在本研究中，我们描述了人冠状病毒229E 3C样蛋白酶在大肠杆菌中的生物合成以及纯化蛋白的底物特异性。利用免疫荧光显微镜，我们还研究了3C样蛋白酶的亚细胞定位，发现该蛋白酶在病毒感染细胞中呈点状、核周分布。
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