Schiller J J, Baker S C
Department of Microbiology and Immunology, Loyola University of Chicago, Stritch School of Medicine, Maywood, Illinois 60153, USA.
Adv Exp Med Biol. 1998;440:135-9. doi: 10.1007/978-1-4615-5331-1_18.
The RNA polymerase gene of the murine coronavirus mouse hepatitis virus (MHV) encodes a polyprotein of greater than 750 kDa. The amino-terminal cleavage product of the MHV polymerase polyprotein, p28, has been shown to be cleaved from the polyprotein by the virus-encoded protease PCP-1. We aim to identify the MHV-JHM proteolytic products downstream of p28 and to determine which viral proteinase domains are responsible for generating each of them. To this end, we have generated antisera directed at specific MHV-JHM ORF1a regions and have used these antisera to identify six viral proteins, representing a large portion of ORF1a, from MHV-JHM-infected cells. These proteins include p28, p72, p65, p250, p210, and p27.
鼠冠状病毒小鼠肝炎病毒（MHV）的RNA聚合酶基因编码一种大于750 kDa的多聚蛋白。MHV聚合酶多聚蛋白的氨基末端裂解产物p28已被证明是由病毒编码的蛋白酶PCP-1从多聚蛋白上裂解下来的。我们旨在鉴定p28下游的MHV-JHM蛋白水解产物，并确定哪些病毒蛋白酶结构域负责产生它们中的每一种。为此，我们制备了针对特定MHV-JHM ORF1a区域的抗血清，并使用这些抗血清从MHV-JHM感染的细胞中鉴定出六种病毒蛋白，它们代表了ORF1a的很大一部分。这些蛋白质包括p28、p72、p65、p250、p210和p27。
