Dalton K, Penzes Z, Wroe C, Stirrups K, Evans S, Shaw K, Brown T D, Britton P, Cavanagh D
IAH, Compton, England.
Adv Exp Med Biol. 1998;440:253-7. doi: 10.1007/978-1-4615-5331-1_32.
Deletion mutagenesis has been used to identify essential regions for rescue of coronavirus defective RNAs (D-RNAs). Using this technique on a cloned IBV D-RNA CD-91, we have identified a region potentially important in its rescue. Comparing the sequence of D-RNAs rescued with those not rescued we have deduced that a 72 base region corresponding to base number 13,824 to 13,896 in the viral genome is required for rescue. This may be an IBV D-RNA packaging signal or a cis-acting element involved in replication. Further experiments and modification of our techniques will be required to differentiate between the two processes.
缺失诱变已被用于鉴定冠状病毒缺陷RNA（D-RNA）拯救的必需区域。在克隆的传染性支气管炎病毒（IBV）D-RNA CD-91上使用该技术，我们已鉴定出一个在其拯救中可能重要的区域。通过比较已拯救和未拯救的D-RNA序列，我们推断病毒基因组中对应于碱基编号13,824至13,896的72个碱基区域是拯救所必需的。这可能是一个IBV D-RNA包装信号或参与复制的顺式作用元件。需要进一步的实验和对我们技术的改进来区分这两个过程。
