分离血凝缺陷型突变体以分析传染性胃肠炎病毒的唾液酸结合活性。

Isolation of hemagglutination-defective mutants for the analysis of the sialic acid binding activity of transmissible gastroenteritis virus.

作者信息

Krempl C, Ballesteros M L, Enjuanes L, Herrler G

机构信息

Institut für Virologie Philipps-Universität Marburg, Germany.

出版信息

Adv Exp Med Biol. 1998;440:563-8. doi: 10.1007/978-1-4615-5331-1_73.

DOI:10.1007/978-1-4615-5331-1_73

PMID:9782330

Abstract

The surface protein S of transmissible gastroenteritis virus (TGEV) has a sialic acid binding activity that enables the virus to agglutinate erythrocytes. A protocol is described that has been successfully applied to the isolation of hemgglutination-defective mutants. The potential of these mutants for the characterization of the sialic acid-binding site and the function of the binding activity is discussed.

摘要

传染性胃肠炎病毒（TGEV）的表面蛋白S具有唾液酸结合活性，可使该病毒凝集红细胞。本文描述了一种已成功应用于分离血凝缺陷型突变体的方法。讨论了这些突变体在表征唾液酸结合位点和结合活性功能方面的潜力。