Collins A R
Department of Microbiology, School of Medicine and Biomedical Sciences, State University of New York at Buffalo 14214, USA.
Adv Exp Med Biol. 1998;440:635-9. doi: 10.1007/978-1-4615-5331-1_82.
Adherent adult and cord blood macrophages were infected with human coronavirus OC43 at a multiplicity of 1-1.5 and washed twice to remove unbound virus. Virus progeny was detected in the supernatant on day 1 and peaked at 2-3 days at an average titer of 5 +/- 3.9 x 10(6) pfu/ml from seven samples. Viral RNA was detected by nested set RT-PCR in infected macrophages incubated for 48 hr. Intracellular viral nucleocapsid was detected in 15% of the cells and surface staining for viral spike antigen was observed using monoclonal antibodies. Amplification of infectious virus and detection viral RNA and antigen synthesis in macrophages in vitro indicates susceptibility to OC43 virus.
贴壁的成人和脐血巨噬细胞以1-1.5的感染复数感染人冠状病毒OC43，然后洗涤两次以去除未结合的病毒。在第1天在上清液中检测到子代病毒，在2-3天达到峰值，七个样本的平均滴度为5 +/- 3.9 x 10(6) pfu/ml。通过巢式RT-PCR在孵育48小时的感染巨噬细胞中检测到病毒RNA。在15%的细胞中检测到细胞内病毒核衣壳，并使用单克隆抗体观察到病毒刺突抗原的表面染色。体外巨噬细胞中感染性病毒的扩增以及病毒RNA和抗原合成的检测表明其对OC43病毒敏感。
