Redbrake C, Frantz A, Salla S
Augenklinik der RWTH Aachen.
Klin Monbl Augenheilkd. 1998 Aug;213(2):93-6. doi: 10.1055/s-2008-1034953.
Metabolic changes of the human donor cornea during organ-culture are not at all reflected by the endothelium. Therefore metabolic investigations have become of increasing interest. It was the aim of this study to determine the correlation between glucose and lactate in storage medium and within the cornea itself and to find thereby an additional parameter for glucose metabolism during organ-culture.
Glucose and lactate were examined in 166 organ-culture medium samples as well as in 106 human corneas by enzymatical optical methods. Investigations were carried out after 1, 7, 15, 21 and 28 days of organ-culture.
Glucose consumption was highest during the first two weeks of organ-culture. Glucose concentrations showed a good linear correlation between medium samples and the cornea (r = 0.923). The correlation coefficient for lactate was worse (r = 0.733).
Glucose and lactate levels in the organ-culture medium can be used as a marker for glucose metabolism in the cornea.
人体供体角膜在器官培养过程中的代谢变化完全没有通过内皮细胞反映出来。因此，代谢研究越来越受到关注。本研究的目的是确定储存培养基和角膜本身中葡萄糖与乳酸之间的相关性，从而找到器官培养期间葡萄糖代谢的一个额外参数。
通过酶光学方法对166份器官培养基样本以及106个人类角膜中的葡萄糖和乳酸进行检测。在器官培养1、7、15、21和28天后进行调查。
器官培养的前两周葡萄糖消耗最高。培养基样本与角膜之间的葡萄糖浓度呈现出良好的线性相关性（r = 0.923）。乳酸的相关系数较差（r = 0.733）。
器官培养基中的葡萄糖和乳酸水平可作为角膜葡萄糖代谢的标志物。
