Klevay L M
J Nutr. 1976 Nov;106(11):1643-6. doi: 10.1093/jn/106.11.1643.
The biotin requirement of rats fed a diet based upon 62.4% sucrose, 20.0% egg white and 10.0% corn oil was determined using growth for 60 days as the cirterion. Maximal growth and maximal concentration of cholesterol in plasma occurred when the diet contained 2.00 mg biotin/kg. Growth depression was noted in one of two experiments in which 3.00 mg of biotin was present per kg of diet.
以60天的生长情况为标准，测定了以62.4%蔗糖、20.0%蛋清和10.0%玉米油为基础日粮喂养的大鼠对生物素的需求量。当日粮中生物素含量为2.00毫克/千克时，大鼠生长达到最大值，血浆中胆固醇浓度也达到最高值。在两项实验中，有一项实验发现，当每千克日粮中生物素含量为3.00毫克时，大鼠生长出现抑制。
