Bril'man Ia E
City Infectious Hospital, Kaliningrad, Russia.
Zh Mikrobiol Epidemiol Immunobiol. 1998 Jul-Aug(4):17-9.
The dehydrogenase (DHG) or oxidation-reduction test is proposed for use together with the determination of such enzymes as hydrolases, cytochrome oxidase, dehydrocarboxylase, urease, etc. 200 Citrobacter freundii cultures and 76 strains of enteropathogenic Escherichia (EPE) were studied with the determination of their DHG activity in semiliquid mannitol and in Kligler's medium. The study revealed that this test, characterized by the reduction of the indicator, similarly to that in salmonellae and shigellae, was constantly negative in semiliquid mannitol in C. freundii and in 97.3% of cases in EPE. In 17.5% of C. freundii lactose-positive cultures the DHG test in Kligler's medium was positive, which made it possible to regard them as a separate biovar. Taking into account the results of this investigation, the subdivision of C. freundii into 3 biovars is proposed.
建议将脱氢酶（DHG）或氧化还原试验与水解酶、细胞色素氧化酶、脱羧酶、脲酶等酶的测定一起使用。对200株弗氏柠檬酸杆菌培养物和76株肠道致病性大肠杆菌（EPE）进行了研究，测定了它们在半液体甘露醇和克氏培养基中的DHG活性。研究表明，该试验以指示剂还原为特征，与沙门氏菌和志贺氏菌中的情况类似，在弗氏柠檬酸杆菌的半液体甘露醇中以及在97.3%的EPE病例中始终呈阴性。在17.5%的乳糖阳性弗氏柠檬酸杆菌培养物中，克氏培养基中的DHG试验呈阳性，这使得有可能将它们视为一个单独的生物变种。考虑到这项研究的结果，建议将弗氏柠檬酸杆菌细分为3个生物变种。
