Kleffmann T, Schmidt J, Schaub G A
Department of Special Zoology, Ruhr University, Bochum, Germany.
J Eukaryot Microbiol. 1998 Sep-Oct;45(5):548-55. doi: 10.1111/j.1550-7408.1998.tb05115.x.
In vivo, epimastigotes of Trypanosoma cruzi colonize a lipidic superficial layer of the rectal cuticle of the vector Triatoma infestans. In vitro, epimastigotes of four cultured strains and one strain from reduviids use a terminal area of the flagellum to attach to a variety of artificial hydrophobic substances, such as hydrocarbons and a range of synthetic plastics. Trypomastigotes did not attach to these substrates. Hydrophilic molecules, such as neutral or negatively charged polysaccharides, did not facilitate binding. Epimastigotes and trypomastigotes were artificially bound by electrostatic forces to positively charged chitosan or DEAE-Sephacel over their entire surface. Tween 20 and lipid-binding serum albumin effectively inhibited the hydrophobic attachment. Based on this hydrophobic interaction of epimastigotes, a new chromatography technique has been devised to gently separate trypomastigotes from epimastigotes using octacosane-coated beads. Furthermore, the in vitro transformation of epimastigotes to trypomastigotes was enhanced if epimastigotes were permitted to attach to hydrophobic, wax-coated culture vessels.
在体内，克氏锥虫的上鞭毛体定殖于传播媒介骚扰锥蝽直肠角质层的脂质表层。在体外，四种培养菌株和一种猎蝽属菌株的上鞭毛体利用鞭毛末端区域附着于多种人工疏水物质，如碳氢化合物和一系列合成塑料。锥鞭毛体不附着于这些底物。亲水分子，如中性或带负电荷的多糖，无助于结合。上鞭毛体和锥鞭毛体通过静电力在其整个表面人工结合到带正电荷的壳聚糖或二乙氨基乙基琼脂糖凝胶上。吐温20和脂质结合血清白蛋白能有效抑制疏水附着。基于上鞭毛体的这种疏水相互作用，已设计出一种新的色谱技术，使用二十八烷包被的珠子从锥鞭毛体中温和分离上鞭毛体。此外，如果允许上鞭毛体附着于疏水的蜡包被培养容器，则上鞭毛体向锥鞭毛体的体外转化会增强。
