Nomura T, Kawakami A, Fujisawa H
Division of Biological Science, Nagoya University Graduate School of Science, Japan.
Dev Growth Differ. 1998 Oct;40(5):485-95. doi: 10.1046/j.1440-169x.1998.t01-3-00003.x.
Heterotopic transplantation of brain vesicles between chick and quail were performed, and the correlation between tectum formation and the expression of two PAX family genes, PAX7 and PAX6, analyzed. Reciprocal transplantation between the prosencephalon and mesencephalon showed that formation of the tectum always coincided with induction/maintenance of PAX7 and suppression of PAX6, indicating that switch-on or -off of these two PAX family genes in region specific manners are responsible for the differentiation of brain vesicles into the tectum. On the other hand, transplantation of the mesencephalic floor plate into the dorsal mesencephalon suppressed PAX7 expression in the dorsal mesencephalon and changed its fate from the tectum to the tegmentum, indicating that factors in the mesencephalic floor plate suppress PAX7 and limit tectum territory to the dorsal part of the mesencephalon.
进行了鸡和鹌鹑之间脑泡的异位移植，并分析了顶盖形成与两个PAX家族基因PAX7和PAX6表达之间的相关性。前脑和中脑之间的相互移植表明，顶盖的形成总是与PAX7的诱导/维持以及PAX6的抑制同时发生，这表明这两个PAX家族基因以区域特异性方式的开启或关闭负责脑泡分化为顶盖。另一方面，将中脑底板移植到中脑背侧会抑制中脑背侧的PAX7表达，并将其命运从顶盖改变为被盖，这表明中脑底板中的因子会抑制PAX7并将顶盖区域限制在中脑的背侧部分。
