Shiokawa S, Yoshimura Y, Nagamatsu S, Sawa H, Hanashi H, Sakai K, Noguchi K, Nakamura Y
Department of Obstetrics and Gynecology, Kyorin University School of Medicine, Mitaka, Tokyo, Japan.
Mol Hum Reprod. 1998 Sep;4(9):907-14. doi: 10.1093/molehr/4.9.907.
The expression and function of focal adhesion kinase (FAK) in human decidual cells were investigated. This kinase is localized to focal adhesions in fibroblasts, and is phosphorylated on tyrosine in normal and src-transformed fibroblasts. Immunofluorescent staining revealed that the cultured decidual cells expressed high levels of FAK at the cell periphery. Double stainings for FAK and phosphotyrosine, FAK and talin, and FAK and beta1 integrin demonstrated that FAK co-localized with integrins in cellular focal adhesions. Mouse blastocysts became attached to cultured decidual cells after embryos hatched from the zona pellucida. The majority of hatched blastocysts attached to human decidual cells within 24 h of culture. Blastocysts attached to decidual cells exhibited extensive outgrowth after 48 h. Treatment of decidual cells with herbimycin A, a tyrosine kinase inhibitor, did not affect the rate of hatching or attachment of blastocysts. However, the outgrowth of embryos on the decidual cells was inhibited by the addition of herbimycin A in a dose-dependent manner, implying that blastocyst attachment and outgrowth are mediated by different mechanisms. This study suggests that tyrosine phosphorylation of FAK on decidual cells may be important in development and differentiation following attachment.
研究了粘着斑激酶（FAK）在人蜕膜细胞中的表达及功能。该激酶定位于成纤维细胞的粘着斑，在正常及src转化的成纤维细胞中酪氨酸位点发生磷酸化。免疫荧光染色显示，培养的蜕膜细胞在细胞周边高表达FAK。FAK与磷酸酪氨酸、FAK与踝蛋白、FAK与β1整合素的双重染色表明，FAK与整合素在细胞粘着斑中共定位。小鼠囊胚从透明带孵化后可附着于培养的蜕膜细胞。大多数孵化后的囊胚在培养24小时内附着于人蜕膜细胞。附着于蜕膜细胞的囊胚在48小时后出现广泛的生长。用酪氨酸激酶抑制剂赫曲霉素A处理蜕膜细胞，不影响囊胚的孵化率或附着率。然而，在蜕膜细胞上添加赫曲霉素A以剂量依赖的方式抑制胚胎的生长，这意味着囊胚附着和生长由不同机制介导。本研究表明，蜕膜细胞上FAK的酪氨酸磷酸化在附着后的发育和分化中可能很重要。
