Neupert G, Glöckner R, Müller D
Institute of Pathology, Friedrich Schiller University Jena, Germany.
Exp Toxicol Pathol. 1998 Sep;50(4-6):514-8. doi: 10.1016/s0940-2993(98)80043-2.
Mono- and polyclonal antibodies have been used to study the localization and distribution of cytochrome P450 1A1 (CYP1A1) in cultured precision-cut liver slices with various immunohistochemical methods. Neither in non-incubated slices nor in slices incubated in the absence of beta-naphthoflavone (BNF) for 24 hrs was CYP1A1 immunohistochemically detectable. After incubation in the presence of BNF (25 microM), however, CYP1A1 was well visible in parenchymal and biliary epithelial cells. CYP1A1 was not evenly distributed, but was localized predominantly in hepatocyte layers near the surfaces of the slices. This distribution could be due to the preferential uptake of BNF by outer cell layers or due to functional changes of inner cells. Together with results obtained with other methods (e.g. RT-PCR) this investigation also demonstrates that precision-cut liver slices are a useful tool for the detection of in vitro induction of CYP1A1.
单克隆抗体和多克隆抗体已被用于通过各种免疫组织化学方法研究细胞色素P450 1A1（CYP1A1）在培养的精密肝切片中的定位和分布。无论是在未孵育的切片中，还是在不存在β-萘黄酮（BNF）的情况下孵育24小时的切片中，均未检测到CYP1A1的免疫组织化学信号。然而，在存在BNF（25微摩尔）的情况下孵育后，CYP1A1在实质细胞和胆管上皮细胞中清晰可见。CYP1A1分布不均匀，主要定位于切片表面附近的肝细胞层。这种分布可能是由于外层细胞优先摄取BNF，或者是由于内层细胞的功能变化。与其他方法（如RT-PCR）获得的结果一起，这项研究还表明，精密肝切片是检测CYP1A1体外诱导的有用工具。
