Yamamoto T, Suzuki S, Radsak K, Hirai K
Department of Cell Regulation, Medical Research Institute, Tokyo Medical and Dental University, Japan.
Virus Res. 1998 Jul;56(1):107-14. doi: 10.1016/s0168-1702(98)00032-x.
The UL112/113 gene products of human cytomegalovirus (HCMV) were shown by transient complementation ori Lyt-dependent DNA replication assay to be early viral proteins required for efficient viral DNA synthesis. By immunofluorescence analysis followed by fluorescence in situ hybridization, we showed that UL112/113 gene products of HCMV are colocalized with viral DNA prior to and during viral DNA replication in infected cell nuclei. We have used an anti-sense RNA approach for functional analysis of the UL112/113 gene in HCMV. The astrocytoma cell line U373-MG was used for permanent expression of the anti-sense UL112/113 gene. Expression of the anti-sense RNA in this cell line significantly blocked expression of UL112/113 gene products and viral DNA replication, indicating that the UL112/113 gene products are related to efficient viral DNA replication.
人巨细胞病毒（HCMV）的UL112/113基因产物通过瞬时互补ori Lyt依赖性DNA复制试验表明是有效病毒DNA合成所需的早期病毒蛋白。通过免疫荧光分析随后进行荧光原位杂交，我们发现HCMV的UL112/113基因产物在感染细胞核中病毒DNA复制之前和期间与病毒DNA共定位。我们已采用反义RNA方法对HCMV中的UL112/113基因进行功能分析。星形细胞瘤细胞系U373-MG用于反义UL112/113基因的永久表达。该细胞系中反义RNA的表达显著阻断了UL112/113基因产物的表达和病毒DNA复制，表明UL112/113基因产物与有效的病毒DNA复制相关。
