Hincha D K
Institut für Pflanzenphysiologie und Mikrobiologie, Freie Universität, Berlin, Königin Luise Strasse 12-16, D-14195, Germany.
Arch Biochem Biophys. 1998 Oct 15;358(2):385-90. doi: 10.1006/abbi.1998.0866.
The dissociation of two peripheral spinach (Spinacia oleracea L.) thylakoid membrane proteins, coupling factor CF1 and ferredoxin-NADP+-oxidoreductase, in the presence of chaotropic sodium salts has been investigated, using monospecific antibodies against the proteins as probes. Release of both proteins followed the Hofmeister series of anions (Cl- < NO3- < Br- < I- < SCN-). In mixtures, the different salts had an additive effect. In addition, there were also qualitative differences in the action of the anions, such that NaI and NaSCN led to a different concentration and time dependence of the dissociation of the peripheral proteins from thylakoids. An analysis of the temperature dependence of protein release showed that the more chaotropic ions reduced the activation energy required for the dissociation of the proteins from their binding sites on the membrane. The addition of sugars (glucose, sucrose, or trehalose) reduced the amount of protein released from the membranes in the presence of NaI or NaSCN.
利用针对这些蛋白质的单特异性抗体作为探针，研究了在离液序列高的钠盐存在下，两种菠菜（Spinacia oleracea L.）外周类囊体膜蛋白——偶联因子CF1和铁氧还蛋白-NADP⁺-氧化还原酶的解离情况。两种蛋白质的释放遵循阴离子的霍夫迈斯特序列（Cl⁻ < NO₃⁻ < Br⁻ < I⁻ < SCN⁻）。在混合物中，不同的盐具有加和效应。此外，阴离子的作用也存在质的差异，使得NaI和NaSCN导致外周蛋白从类囊体解离的浓度和时间依赖性不同。对蛋白质释放的温度依赖性分析表明，离液序列更高的离子降低了蛋白质从膜上结合位点解离所需的活化能。在NaI或NaSCN存在的情况下，添加糖类（葡萄糖、蔗糖或海藻糖）减少了从膜上释放的蛋白量。
