Pombo V, Velloso LM, Ribeiro BM, Bao SN
Departamento de Biologia Celular, Universidade de Brasilia, Brasilia DF, 70919-970, Brazil.
J Invertebr Pathol. 1998 Nov;72(3):239-45. doi: 10.1006/jipa.1998.4788.
During infection of the permissive insect cell line UFL-AG-286 by the baculovirus Anticarsia gemmatalis nucleopolyhedrovirus (AgMNPV-2D) several morphological changes occur. By 12 h postinfection (h p.i.), the infected cells became round and exhibited a decrease in the number of cytoplasmic projections. By 24 h p.i., it was possible to detect a virogenic stroma inside the cell nucleus, and after 48 h p.i., polyhedral inclusion bodies were observed. Some of these morphological modifications are probably due to changes in the cytoskeleton of the cell and this possibility was substantiated by the observation that the distribution of actin and microtubules was dramatically modified upon infection. Several viral-induced proteins were also produced during infection and a sharp decrease in overall protein synthesis was observed. These results are very similar to those obtained with other cell lines infected with different baculoviruses, indicating a similar mechanism of infection. Copyright 1998 Academic Press.
在杆状病毒斜纹夜蛾核型多角体病毒（AgMNPV - 2D）感染敏感昆虫细胞系UFL - AG - 286的过程中，会发生几种形态学变化。感染后12小时（h p.i.），被感染的细胞变成圆形，细胞质突起数量减少。感染后24小时，可以在细胞核内检测到病毒发生基质，感染后48小时，观察到多面体包涵体。这些形态学改变中的一些可能是由于细胞骨架的变化，并且通过观察到感染后肌动蛋白和微管的分布发生显著改变，这一可能性得到了证实。感染期间还产生了几种病毒诱导蛋白，并且观察到总蛋白合成急剧下降。这些结果与用感染不同杆状病毒的其他细胞系获得的结果非常相似，表明存在相似的感染机制。版权所有1998年学术出版社。
