Tokunaga K, Kojima A, Kurata T, Ikuta K, Inubushi R, Shimano R, Kawamura M, Akari H, Koyama A H, Adachi A
Department of Pathology, National Institute of Infectious Diseases, Tokyo, 162-8640, Japan.
Biochem Biophys Res Commun. 1998 Sep 29;250(3):565-8. doi: 10.1006/bbrc.1998.9346.
A proviral nef gene mutant of human immunodeficiency virus type 1 (HIV-1) was evaluated for its defective early replication step. Virus stocks were prepared from six CD4-positive and -negative cell lines transfected with wild-type (wt) or the nef mutant clone and inoculated into two target CD4-positive cell lines to monitor the efficiency of viral entry process. The nef mutant virions produced in one cell line exhibited a severe defect in the entry process, although those produced in the other five cell lines were only slightly less efficient than the wt virions at entering into cells. These results have demonstrated that the HIV-1 Nef is critical for efficient viral entry in a producer cell-dependent manner.
对1型人类免疫缺陷病毒（HIV-1）的前病毒nef基因变体进行了评估，以研究其早期复制步骤的缺陷情况。从转染了野生型（wt）或nef突变体克隆的六种CD4阳性和阴性细胞系中制备病毒储备液，并将其接种到两种靶CD4阳性细胞系中，以监测病毒进入过程的效率。在一个细胞系中产生的nef突变体病毒粒子在进入过程中表现出严重缺陷，尽管在其他五个细胞系中产生的病毒粒子进入细胞的效率仅略低于野生型病毒粒子。这些结果表明，HIV-1 Nef以依赖产生细胞的方式对病毒有效进入至关重要。
