Le Petit-Thevenin J, Pasqualini E, Nobili O, Vérine A, Lombardo D
INSERM U260, Faculté de Médecine-Timone, 27 Blv. Jean Moulin, 13385 Marseille Cedex 5, France.
Biochim Biophys Acta. 1998 Oct 22;1408(1):44-54. doi: 10.1016/s0925-4439(98)00054-4.
The mechanisms by which ethanol administration alters pancreatic function are unknown. We have evaluated the effects of chronic ethanol treatment on secretion of a digestive enzyme: the bile salt-dependent lipase (BSDL), by the rat pancreatic cell line AR4-2J (as a model). We report that ethanol (50-300 mM) in culture medium induced a rise, in secreted and intracellular BSDL, that was a function of the duration of treatment and of the ethanol concentration. This effect was not abolished by pyrazole, which suggests a direct effect of ethanol. We have further established that the increase of BSDL activity was due to an enhanced biosynthesis of the enzyme consecutive to a major steady-state level of mRNA encoding BSDL. Also, the subcellular localization showed a specific accumulation of BSDL in the cytosolic fraction of cells chronically treated with ethanol. Given the enzymatic properties of BSDL, all these data could have some physiological consequences regarding the digestive function, plasma lipid metabolism and intracellular cholesterol homeostasis.
乙醇摄入改变胰腺功能的机制尚不清楚。我们已评估慢性乙醇处理对大鼠胰腺细胞系AR4-2J（作为模型）分泌一种消化酶——胆汁盐依赖性脂肪酶（BSDL）的影响。我们报告，培养基中的乙醇（50 - 300 mM）诱导分泌型和细胞内BSDL增加，这是处理持续时间和乙醇浓度的函数。吡唑并未消除这种作用，这表明乙醇具有直接作用。我们进一步确定，BSDL活性的增加是由于编码BSDL的mRNA主要稳态水平之后该酶的生物合成增强所致。此外，亚细胞定位显示，在长期用乙醇处理的细胞的胞质部分中，BSDL有特异性积累。鉴于BSDL的酶学特性，所有这些数据可能对消化功能、血浆脂质代谢和细胞内胆固醇稳态有一些生理影响。
