金属卟啉介导的蛋白质交联
Protein cross-linking mediated by metalloporphyrins.
作者信息
Campbell L A, Kodadek T, Brown K C
机构信息
Department of Chemistry and Biochemistry, University of Texas, Austin 78712, USA.
出版信息
Bioorg Med Chem. 1998 Aug;6(8):1301-7. doi: 10.1016/s0968-0896(98)00120-5.
PMID:9784871
Abstract
A biomimetic protein cross-linking reaction is described which employs oxidatively-activated manganese and iron porphyrins as the reactive species. A wide range of proteins cross-link under these conditions, but only if they are intimately associated in solution. The reaction is rapid, efficient, and will be useful for the suprastructural analysis of multiprotein complexes.
摘要
描述了一种仿生蛋白质交联反应，该反应采用氧化活化的锰卟啉和铁卟啉作为反应活性物质。在这些条件下，多种蛋白质会发生交联，但前提是它们在溶液中紧密缔合。该反应快速、高效，将有助于多蛋白复合物的超结构分析。
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