Campbell L A, Kodadek T, Brown K C
Department of Chemistry and Biochemistry, University of Texas, Austin 78712, USA.
Bioorg Med Chem. 1998 Aug;6(8):1301-7. doi: 10.1016/s0968-0896(98)00120-5.
A biomimetic protein cross-linking reaction is described which employs oxidatively-activated manganese and iron porphyrins as the reactive species. A wide range of proteins cross-link under these conditions, but only if they are intimately associated in solution. The reaction is rapid, efficient, and will be useful for the suprastructural analysis of multiprotein complexes.
描述了一种仿生蛋白质交联反应，该反应采用氧化活化的锰卟啉和铁卟啉作为反应活性物质。在这些条件下，多种蛋白质会发生交联，但前提是它们在溶液中紧密缔合。该反应快速、高效，将有助于多蛋白复合物的超结构分析。
