小鼠着床部位胰岛素样生长因子结合蛋白-1、-2和-3的免疫组织化学分析
Immunohistochemical analysis of insulin-like growth factor-binding proteins -1, -2, and -3 in implantation sites of the mouse.
作者信息
Damario M A, Liu H C, Mele C A, Horenstein M G, Rosenwaks Z
机构信息
Department of Obstetrics and Gynecology, Cornell University Medical College, New York, New York, USA.
出版信息
J Assist Reprod Genet. 1998 Sep;15(8):513-20. doi: 10.1023/a:1022538621928.
PURPOSE
Our purpose was to analyze potential interactions between the embryo and the maternal endometrial interface in vivo by analyzing immunolocalization of insulin-like growth factor-binding proteins (IGFBPs) -1, -2, and -3 in implantation sites of the mouse.
METHODS
Six-week-old B6D2F1 female mice underwent superovulation followed by mating and sacrifice at timed intervals. Formalin-fixed paraffin-embedded tissue was used for avidin-biotin immunocytochemical localization of IGFBPs utilizing standard methodology.
RESULTS
Immunostaining at 1.5 days post coitum revealed light staining in the epithelial glandular cells and faint staining in decidual stroma for both IGFBP-1 and IGFBP-2. At 7.5-10.5 days post coitum, there was moderate-dense immunostaining in the decidualized stromal cells at the implantation site for all three IGFBPs, whereas light immunostaining was seen in nonimplantation site decidua.
CONCLUSIONS
Compartmentalization of immunostaining for IGFBP-1, -2, and -3 within decidualized stroma suggests that these proteins may be regulated by trophoblastic and/or embryonic signals.
目的
我们的目的是通过分析胰岛素样生长因子结合蛋白（IGFBPs）-1、-2和-3在小鼠着床部位的免疫定位，来分析胚胎与母体子宫内膜界面之间在体内的潜在相互作用。
方法
6周龄的B6D2F1雌性小鼠进行超排卵，随后交配，并在特定时间间隔处牺牲。使用标准方法，将福尔马林固定石蜡包埋的组织用于IGFBPs的抗生物素蛋白-生物素免疫细胞化学定位。
结果
在交配后1.5天，IGFBP-1和IGFBP-2在上皮腺细胞中呈浅染色，在蜕膜基质中呈淡染色。在交配后7.5 - 10.5天，所有三种IGFBPs在着床部位的蜕膜化基质细胞中呈中度密集免疫染色，而在未着床部位的蜕膜中可见浅免疫染色。
结论
蜕膜化基质中IGFBP-1、-2和-3免疫染色的分区表明，这些蛋白质可能受滋养层和/或胚胎信号调控。
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