Veremeĭchenko S N
Institute of Microbiology and Virology, National Academy of Sciences of Ukraine, Kiev, Ukraine.
Mikrobiologiia. 1998 Jul-Aug;67(4):505-11.
The lipopolysaccharide of strain Pseudomonas fluorescens IMV 1433 (biovar I) was isolated and investigated. 3-Hydroxydecanoic, 2-hydroxydodecanoic, 3-hydroxydodecanoic, dodecanoic, hexadecanoic, hexadecenoic, and octadecenoic acids were identified in the composition of lipid A. The total amount of hydroxy acids makes up 60%. In the hydrolysate of lipid A, components of the were revealed hydrophilic skeleton--glucosamine and phosphoethanolamine--were revealed. Glucose, galactose, rhamnose, glucosamine, galactosamine, alanine, phosphoethanolamine, phosphorus, and 2-keto-3-deoxyoctulosonic acid were identified in the core of the lipopolysaccharide molecule. O-specific polysaccharide chains of the lipopolysaccharide are built from repeating trisaccharide units consisting of the residues of 2-acetamido-2,6-dideoxy-L-glucose, 2-acetamido-2,6-dideoxy-D-glucose and 4-acetamido-4,6-dideoxy-D-galactose. The lipopolysaccharide studied is similar to the lipopolysaccharide of P. fluorescens IMV 1152 (biovar I) in the structure of lipid A and core oligosaccharide, and identical to it in the structure of the O-chain (this identity correlates with the high level of the serological relationship of the strains).
对荧光假单胞菌IMV 1433（生物变种I）菌株的脂多糖进行了分离和研究。在类脂A的组成中鉴定出了3-羟基癸酸、2-羟基十二烷酸、3-羟基十二烷酸、十二烷酸、十六烷酸、十六碳烯酸和十八碳烯酸。羟基酸的总量占60%。在类脂A的水解产物中，发现了其亲水骨架的成分——葡糖胺和磷酸乙醇胺。在脂多糖分子的核心中鉴定出了葡萄糖、半乳糖、鼠李糖、葡糖胺、半乳糖胺、丙氨酸、磷酸乙醇胺、磷和2-酮-3-脱氧辛糖酸。脂多糖的O-特异性多糖链由重复的三糖单元构成，这些单元由2-乙酰氨基-2,6-二脱氧-L-葡萄糖、2-乙酰氨基-2,6-二脱氧-D-葡萄糖和4-乙酰氨基-4,6-二脱氧-D-半乳糖的残基组成。所研究的脂多糖在类脂A和核心寡糖的结构上与荧光假单胞菌IMV 1152（生物变种I）的脂多糖相似，在O链的结构上与之相同（这种相同性与菌株的高血清学关系水平相关）。
