Maree S, Durbach S, Maree F F, Vreede F, Huismans H
Biochemistry Division, Onderstepoort Veterinary Institute, South Africa.
Arch Virol Suppl. 1998;14:203-9. doi: 10.1007/978-3-7091-6823-3_18.
The genome segments encoding the seven structural proteins of African horse sickness virus (AHSV), including the largest coding for VP1, were cloned and sequenced. Analysis of the VP1 sequence supports the putative identity of this protein as an RNA polymerase. The genes encoding the two major core proteins, VP3 and VP7, were also cloned and expressed by both in vitro translation and by means of recombinant baculoviruses. Co-infection of insect cells with VP3 and VP7 recombinant baculoviruses resulted in the intracellular formation of multimeric particles with a diameter of 72 nm, which structurally resembled authentic AHSV cores (core like particles: CLP). The complete genome of AHSV has now been cloned and sequenced.
编码非洲马瘟病毒（AHSV）七种结构蛋白的基因组片段被克隆并测序，其中编码最大蛋白VP1的片段也被克隆和测序。对VP1序列的分析支持了该蛋白作为RNA聚合酶的假定身份。编码两种主要核心蛋白VP3和VP7的基因也通过体外翻译和重组杆状病毒进行了克隆和表达。用VP3和VP7重组杆状病毒共同感染昆虫细胞，导致细胞内形成直径为72nm的多聚体颗粒，其结构类似于正宗的AHSV核心（核心样颗粒：CLP）。AHSV的完整基因组现已被克隆和测序。
