Otani M, Kozuka S, Xu C, Umezawa C, Sano K, Inouye S
The Faculty of Pharmaceutical Science, Kobe-Gakuin University, Kobe, Japan.
Mol Microbiol. 1998 Oct;30(1):57-66. doi: 10.1046/j.1365-2958.1998.01037.x.
The gene for the major spore-specific protein, termed protein W, was cloned, and it was found that protein W is composed of 426 amino acid residues including 31% charged (133 residues) and 39% hydrophobic (166 residues) amino acids. In the protein, a motif consisting of five amino acid residues [(V/L/I)-R-E-R-(V/L/I)] is repeated 28 times, and another motif [M-M-(P/G)-Q-G] five times. Protein W is synthesized during a very late stage of development, forming a single, large electron-dense particle (200-400 nm in diameter) inside a spore. X-ray microanalysis of the particle revealed that it contains a high amount of phosphate in addition to calcium and magnesium. It is proposed that protein W consisting of highly charged repetitive sequences is a polyphosphate storage protein to store energy in spores. The disruption of the gene for protein W resulted in delayed fruiting body formation and a lower spore yield.
克隆了编码主要孢子特异性蛋白（称为蛋白W）的基因，发现蛋白W由426个氨基酸残基组成，其中31%为带电荷氨基酸（133个残基），39%为疏水氨基酸（166个残基）。在该蛋白中，由五个氨基酸残基[(V/L/I)-R-E-R-(V/L/I)]组成的基序重复出现28次，另一个基序[M-M-(P/G)-Q-G]重复出现五次。蛋白W在发育的非常后期合成，在孢子内形成单个大的电子致密颗粒（直径200 - 400 nm）。对该颗粒的X射线微分析表明，除钙和镁外，它还含有大量的磷酸盐。有人提出，由高度带电荷的重复序列组成的蛋白W是一种多磷酸盐储存蛋白，用于在孢子中储存能量。蛋白W基因的破坏导致子实体形成延迟和孢子产量降低。
