von Euler M, Larsen J O, Janson A M
Department of Clinical Neuroscience and Family Medicine, Karolinska Institute, Huddinge University Hospital, Sweden.
J Comp Neurol. 1998 Nov 2;400(4):441-8.
Spontaneous reocurrence of neurofilament (NF)-positive fibers has been described after spinal cord lesions in rats. However, previously introduced methods to evaluate the lesion and the regenerative fiber outgrowth suffer from several biases, why a new concept of quantitative, morphological analysis after spinal cord injury is needed. Length quantification of the putatively spontaneously regenerating fibers has been difficult until recently, when two length estimators based on sampling with isotropic virtual planes within thick physical sections were introduced. The applicability of these techniques to estimate the total length of NF-positive fibers was evaluated in photochemically induced ischemic lesions of thoracic spinal cords in young rats 6 weeks postlesion. Fiber length was found to be the most consistent measure with a mean of 3.71 m (coefficient of variation, CV = 0.16) in the 0.90 mm3 (CV = 0.26) large lesions. Whether or not the NF-positive fibers observed inside the lesion represent spontaneously regenerating axons needs to be confirmed in longitudinal, functional, and ultrastructural studies.
大鼠脊髓损伤后神经丝（NF）阳性纤维的自发再出现已有报道。然而，先前用于评估损伤和再生纤维生长的方法存在若干偏差，因此需要一种脊髓损伤后定量形态学分析的新概念。直到最近，当引入基于在厚物理切片内用各向同性虚拟平面采样的两种长度估计器时，对假定自发再生纤维的长度进行定量一直很困难。在损伤后6周的幼鼠胸段脊髓光化学诱导缺血性损伤中，评估了这些技术估计NF阳性纤维总长度的适用性。在0.90 mm³（变异系数，CV = 0.26）的大损伤中，发现纤维长度是最一致的测量指标，平均为3.71 m（变异系数，CV = 0.16）。损伤内观察到的NF阳性纤维是否代表自发再生轴突，需要在纵向、功能和超微结构研究中得到证实。
