Ferragamo M J, Golding N L, Gardner S M, Oertel D
Department of Physiology, University of Wisconsin-Madison Medical School, 53706-1532, USA.
J Comp Neurol. 1998 Nov 2;400(4):519-28.
Golgi cells are poised to integrate multimodal influences by participating in circuits involving granule cells in the cochlear nuclei. To understand their physiological role, intracellular recordings were made from anatomically identified Golgi cells in slices of the cochlear nuclei from mice. Cell bodies, dendrites, and terminals for all seven labeled cells were restricted to the narrow plane of the superficial granule cell domain over the ventral cochlear nucleus. The axonal arborization was the most striking feature of all Golgi cells; a dense plexus of terminals covered an area 200-400 microm in diameter in the vicinity of the cell body and dendrites. Axonal beads often surrounded granule cell bodies, indicating that granule cells are probable targets. Cells had input resistances up to 130 M omega and fired regular, overshooting action potentials. Golgi cells probably receive auditory nerve input, because shocks to the cut end of the auditory nerve excited Golgi cells with excitatory postsynaptic potentials (EPSPs). The latency of EPSPs shortened to a minimum and the amplitude of EPSPs grew in several steps as the strength of shocks was increased. The minimum latency of EPSPs in Golgi cells was on average 1.3 milliseconds, 0.6 milliseconds longer than the minimum latencies of EPSPs in nearby octopus and T stellate cells. The long latency raises the possibility that Golgi cells receive input from slowly conducting, unmyelinated auditory nerve fibers. Golgi cells are also excited by interneurons with N-methyl-D-aspartate receptors, probably granule cells, because repetitive shocks and single shocks in the absence of extracellular Mg2+ evoked late EPSPs that were reversibly blocked by DL-2-amino-5-phosphono-valeric acid.
高尔基细胞通过参与涉及耳蜗核颗粒细胞的回路来整合多模式影响。为了解它们的生理作用，对来自小鼠耳蜗核切片中经解剖学鉴定的高尔基细胞进行了细胞内记录。所有七个标记细胞的胞体、树突和终末都局限于腹侧耳蜗核上方浅表颗粒细胞区域的狭窄平面内。轴突分支是所有高尔基细胞最显著的特征；终末的密集丛覆盖了胞体和树突附近直径200 - 400微米的区域。轴突小珠常常围绕着颗粒细胞胞体，表明颗粒细胞可能是其靶细胞。细胞的输入电阻高达130兆欧，并产生规则的、超射动作电位。高尔基细胞可能接受听神经输入，因为对听神经切断端的电刺激以兴奋性突触后电位（EPSP）激发高尔基细胞。随着刺激强度增加，EPSP的潜伏期缩短至最小值，且EPSP的幅度分几步增大。高尔基细胞中EPSP的最小潜伏期平均为1.3毫秒，比附近章鱼状细胞和T星状细胞中EPSP的最小潜伏期长0.6毫秒。较长的潜伏期增加了高尔基细胞接受来自传导缓慢、无髓鞘听神经纤维输入的可能性。高尔基细胞也受到具有N - 甲基 - D - 天冬氨酸受体的中间神经元的兴奋，可能是颗粒细胞，因为在没有细胞外镁离子的情况下，重复性刺激和单次刺激会诱发晚期EPSP，而这些晚期EPSP可被DL - 2 - 氨基 - 5 - 膦酸戊酸可逆性阻断。
