细胞骨架组装和ATP释放调节星形胶质细胞的钙信号传导。
Cytoskeletal assembly and ATP release regulate astrocytic calcium signaling.
作者信息
Cotrina M L, Lin J H, Nedergaard M
机构信息
Departments of Cell Biology and Anatomy, Pathology, and Neurosurgery, New York Medical College, Valhalla, New York 10595, USA.
出版信息
J Neurosci. 1998 Nov 1;18(21):8794-804. doi: 10.1523/JNEUROSCI.18-21-08794.1998.
Abstract
We have studied the role of actin fiber assembly on calcium signaling in astrocytes. We found that (1) after astrocytes have been placed in culture, it takes several hours for organization of the definitive actin cytoskeleton. Actin organization and the number of cells engaged in calcium signaling increased in parallel. (2) Disruption of the actin cytoskeleton attenuated the calcium wave propagation; cytochalasin D treatment reduced the number of astrocytes engaged in calcium signaling. (3) Propagation of calcium waves depends on cytoskeletal function; inhibition of myosin light chain kinase suppressed wave activity. (4) Astrocytic calcium signaling is mediated by release of ATP and purinergic receptor stimulation, because agents that interfere with this cascade attenuated or reduced calcium signaling. Because purinergic receptors are fully functional shortly after plating and not affected by cytochalasin D, these observations indicate that cytoskeleton organization is a prerequisite for interastrocytic calcium signaling mediated by release of ATP.
摘要
我们研究了肌动蛋白纤维组装在星形胶质细胞钙信号传导中的作用。我们发现：（1）星形胶质细胞接种于培养物后，需要数小时才能形成确定的肌动蛋白细胞骨架。肌动蛋白组织和参与钙信号传导的细胞数量呈平行增加。（2）肌动蛋白细胞骨架的破坏减弱了钙波传播；细胞松弛素D处理减少了参与钙信号传导的星形胶质细胞数量。（3）钙波的传播依赖于细胞骨架功能；肌球蛋白轻链激酶的抑制抑制了波活动。（4）星形胶质细胞的钙信号传导由ATP释放和嘌呤能受体刺激介导，因为干扰该级联反应的试剂会减弱或减少钙信号传导。由于嘌呤能受体在接种后不久就完全发挥功能且不受细胞松弛素D影响，这些观察结果表明细胞骨架组织是由ATP释放介导的星形胶质细胞间钙信号传导的先决条件。
相似文献
1
Cytoskeletal assembly and ATP release regulate astrocytic calcium signaling.细胞骨架组装和ATP释放调节星形胶质细胞的钙信号传导。
J Neurosci. 1998 Nov 1;18(21):8794-804. doi: 10.1523/JNEUROSCI.18-21-08794.1998.
2
Intercellular communication in spinal cord astrocytes: fine tuning between gap junctions and P2 nucleotide receptors in calcium wave propagation.脊髓星形胶质细胞中的细胞间通讯：钙波传播中缝隙连接与P2核苷酸受体之间的精细调节。
J Neurosci. 2000 Feb 15;20(4):1435-45. doi: 10.1523/JNEUROSCI.20-04-01435.2000.
3
Disruption of actin cytoskeleton in cultured rat astrocytes suppresses ATP- and bradykinin-induced [Ca(2+)](i) oscillations by reducing the coupling efficiency between Ca(2+) release, capacitative Ca(2+) entry, and store refilling.培养的大鼠星形胶质细胞中肌动蛋白细胞骨架的破坏通过降低钙释放、钙库调控性钙内流和钙库再充盈之间的偶联效率，抑制了ATP和缓激肽诱导的细胞内钙离子浓度（[Ca(2+)](i)）振荡。
Neuroscience. 2000;97(4):765-9. doi: 10.1016/s0306-4522(00)00062-2.
4
Activity-dependent ATP-waves in the mouse neocortex are independent from astrocytic calcium waves.小鼠新皮质中依赖活动的ATP波独立于星形胶质细胞钙波。
Cereb Cortex. 2006 Feb;16(2):237-46. doi: 10.1093/cercor/bhi101. Epub 2005 Jun 1.
5
Vesicular ATP is the predominant cause of intercellular calcium waves in astrocytes.囊泡ATP是星形胶质细胞中细胞间钙波的主要成因。
J Gen Physiol. 2007 Jun;129(6):485-91. doi: 10.1085/jgp.200709780. Epub 2007 May 15.
6
GAP junctional channel inhibition alters actin organization and calcium propagation in rat cultured astrocytes.缝隙连接通道抑制改变大鼠培养星形胶质细胞中的肌动蛋白组织和钙传播。
Neuroscience. 2002;112(3):593-603. doi: 10.1016/s0306-4522(02)00095-7.
7
Differential properties of astrocyte calcium waves mediated by P2Y1 and P2Y2 receptors.由P2Y1和P2Y2受体介导的星形胶质细胞钙波的差异特性。
J Neurosci. 2003 Jul 30;23(17):6728-39. doi: 10.1523/JNEUROSCI.23-17-06728.2003.
8
Photoliberating inositol-1,4,5-trisphosphate triggers ATP release that is blocked by the connexin mimetic peptide gap 26.光释放肌醇-1,4,5-三磷酸会引发三磷酸腺苷（ATP）释放，而连接蛋白模拟肽gap 26可阻断这种释放。
Cell Calcium. 2003 Jan;33(1):37-48. doi: 10.1016/s0143-4160(02)00180-x.
9
Propagation of intercellular calcium waves in retinal astrocytes and Müller cells.视网膜星形胶质细胞和米勒细胞中细胞间钙波的传播。
J Neurosci. 2001 Apr 1;21(7):2215-23. doi: 10.1523/JNEUROSCI.21-07-02215.2001.
10
Adenosine opposes thrombin-induced inhibition of intercellular calcium wave in corneal endothelial cells.腺苷可对抗凝血酶诱导的角膜内皮细胞细胞间钙波抑制作用。
Invest Ophthalmol Vis Sci. 2007 Apr;48(4):1518-27. doi: 10.1167/iovs.06-1062.
引用本文的文献
1
Viroporin activity is necessary for intercellular calcium signals that contribute to viral pathogenesis.病毒孔蛋白活性对于促成病毒发病机制的细胞间钙信号传导而言是必需的。
Sci Adv. 2025 Jan 17;11(3):eadq8115. doi: 10.1126/sciadv.adq8115.
2
Shared patterns of glial transcriptional dysregulation link Huntington's disease and schizophrenia.亨廷顿病和精神分裂症的神经胶质转录失调存在共同模式。
Brain. 2024 Sep 3;147(9):3099-3112. doi: 10.1093/brain/awae166.
3
Nanotopography and Microconfinement Impact on Primary Hippocampal Astrocyte Morphology, Cytoskeleton and Spontaneous Calcium Wave Signalling.纳米形貌和微限制对原代海马星形胶质细胞形态、细胞骨架和自发性钙波信号的影响。
Cells. 2023 Jan 12;12(2):293. doi: 10.3390/cells12020293.
4
The Effects of Optogenetic Activation of Astrocytes on Spike-and-Wave Discharges in Genetic Absence Epileptic Rats.星形胶质细胞光遗传学激活对遗传性失神癫痫大鼠棘波-慢波放电的影响
Ann Neurosci. 2022 Jan;29(1):53-61. doi: 10.1177/09727531211072423. Epub 2022 Mar 14.
5
Far infrared light irradiation enhances Aβ clearance via increased exocytotic microglial ATP and ameliorates cognitive deficit in Alzheimer's disease-like mice.远红外光照射通过增加细胞外突的小胶质细胞 ATP 来增强 Aβ 的清除，改善阿尔茨海默病样小鼠的认知缺陷。
J Neuroinflammation. 2022 Jun 14;19(1):145. doi: 10.1186/s12974-022-02521-y.
6
Extracellular Calcium Ion Concentration Regulates Chondrocyte Elastic Modulus and Adhesion Behavior.细胞外钙离子浓度调节软骨细胞的弹性模量和黏附行为。
Int J Mol Sci. 2021 Sep 17;22(18):10034. doi: 10.3390/ijms221810034.
7
Astroglial Regulation of Magnocellular Neuroendocrine Cell Activities in the Supraoptic Nucleus.星形胶质细胞对神经垂体大细胞神经内分泌细胞活动的调节。
Neurochem Res. 2021 Oct;46(10):2586-2600. doi: 10.1007/s11064-020-03172-2. Epub 2020 Nov 20.
8
Neonatal Seizures and Purinergic Signalling.新生儿惊厥与嘌呤能信号转导
Int J Mol Sci. 2020 Oct 22;21(21):7832. doi: 10.3390/ijms21217832.
9
The Importance and Control of Low-Grade Inflammation Due to Damage of Cellular Barrier Systems That May Lead to Systemic Inflammation.细胞屏障系统损伤所致低度炎症的重要性及其控制，该炎症可能导致全身炎症。
Front Neurol. 2019 May 29;10:533. doi: 10.3389/fneur.2019.00533. eCollection 2019.
10
Beyond drug-induced alteration of glutamate homeostasis, astrocytes may contribute to dopamine-dependent intrastriatal functional shifts that underlie the development of drug addiction: A working hypothesis.除了药物引起的谷氨酸稳态改变之外，星形胶质细胞可能有助于多巴胺依赖性纹状体内部功能转移，而后者是药物成瘾发展的基础：一个工作假说。
Eur J Neurosci. 2019 Sep;50(6):3014-3027. doi: 10.1111/ejn.14416. Epub 2019 May 14.
本文引用的文献
1
Glutamate-dependent astrocyte modulation of synaptic transmission between cultured hippocampal neurons.谷氨酸依赖的星形胶质细胞对培养海马神经元间突触传递的调节
Eur J Neurosci. 1998 Jun;10(6):2129-42. doi: 10.1046/j.1460-9568.1998.00221.x.
2
Calcium waves between astrocytes from Cx43 knockout mice.来自Cx43基因敲除小鼠的星形胶质细胞之间的钙波。
Glia. 1998 Sep;24(1):65-73. doi: 10.1002/(sici)1098-1136(199809)24:1<65::aid-glia7>3.0.co;2-#.
3
Modulation of neuronal activity by glial cells in the retina.视网膜中神经胶质细胞对神经元活动的调节
J Neurosci. 1998 Jun 1;18(11):4022-8. doi: 10.1523/JNEUROSCI.18-11-04022.1998.
4
Astrocytic gap junctions remain open during ischemic conditions.在缺血状态下，星形胶质细胞间隙连接保持开放。
J Neurosci. 1998 Apr 1;18(7):2520-37. doi: 10.1523/JNEUROSCI.18-07-02520.1998.
5
Intercellular calcium signaling via gap junction in connexin-43-transfected cells.通过连接蛋白43转染细胞中的间隙连接进行的细胞间钙信号传导。
J Biol Chem. 1998 Jan 16;273(3):1519-28. doi: 10.1074/jbc.273.3.1519.
6
ATP- and gap junction-dependent intercellular calcium signaling in osteoblastic cells.成骨细胞中依赖于三磷酸腺苷（ATP）和间隙连接的细胞间钙信号传导
J Cell Biol. 1997 Oct 20;139(2):497-506. doi: 10.1083/jcb.139.2.497.
7
Intracellular calcium oscillations in astrocytes: a highly plastic, bidirectional form of communication between neurons and astrocytes in situ.星形胶质细胞内的钙振荡：神经元与原位星形胶质细胞之间一种高度可塑性的双向通讯形式。
J Neurosci. 1997 Oct 15;17(20):7817-30. doi: 10.1523/JNEUROSCI.17-20-07817.1997.
8
Altered gap junctional communication, intercellular signaling, and growth in cultured astrocytes deficient in connexin43.在缺乏连接蛋白43的培养星形胶质细胞中，缝隙连接通讯、细胞间信号传导及生长发生改变。
J Neurosci Res. 1997 Sep 1;49(5):528-40. doi: 10.1002/(SICI)1097-4547(19970901)49:5<528::AID-JNR3>3.0.CO;2-D.
9
Characterization of the Ca2+ responses evoked by ATP and other nucleotides in mammalian brain astrocytes.哺乳动物脑星形胶质细胞中ATP及其他核苷酸引发的Ca2+反应的特征分析。
Br J Pharmacol. 1997 Aug;121(8):1700-6. doi: 10.1038/sj.bjp.0701293.
10
Microtubule-microfilament synergy in the cytoskeleton.细胞骨架中的微管-微丝协同作用。
Int Rev Cytol. 1997;173:207-42. doi: 10.1016/s0074-7696(08)62478-x.
Zotero 插件