具有骨诱导活性的天然犬骨形态发生蛋白（cBMP）的提取与表征。
Extraction and characterization of native canine bone morphogenetic protein (cBMP) qualified with osteoinductivity.
作者信息
Oksanen J, Marttinen A, Paatsama S, Lindholm T S
机构信息
Surgical Bioimplants Center, University of Tampere, Finland.
出版信息
Acta Vet Scand. 1998;39(2):165-71. doi: 10.1186/BF03547789.
Bone morphogenetic protein (BMP) was extracted from canine bone matrix, partially purified and tested for osteoinductivity. A radiographically and histologically detectable ectopic bone formation was induced by 6.0 mg canine (cBMP) in muscle pouch of BALB mouse at 21 days post implantation. Characterization of the cBMP preparation by a gel filtration chromatography defined that the material consisted of proteins or protein complexes with molecular weights from 4 to 120 kD. Isoelectric focusing showed that the molecules were acidic with isoelectric points of 4.6-5.6.
骨形态发生蛋白（BMP）从犬骨基质中提取，经过部分纯化并检测其骨诱导活性。在植入后21天，6.0毫克犬骨形态发生蛋白（cBMP）在BALB小鼠的肌袋中诱导出了可通过放射学和组织学检测到的异位骨形成。通过凝胶过滤色谱法对cBMP制剂进行表征，确定该物质由分子量为4至120 kD的蛋白质或蛋白质复合物组成。等电聚焦显示这些分子呈酸性，等电点为4.6 - 5.6。
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