Shimamoto I, Sonoda S, Vazquez P, Minaka N, Nishiguchi M
National Institute of Agrobiological Resources, Ibaraki, Japan.
Arch Virol. 1998;143(9):1801-13. doi: 10.1007/s007050050418.
The 3' terminal 2378 nucleotides of a wasabi strain of crucifer tobamovirus (CTMV-W) infectious to crucifer plants was determined. This includes the 3' non-coding region of 235 nucleotides, coat protein (CP) gene (468 nucleotides), movement protein (MP) gene (798 nucleotides) and C-terminal partial readthrough portion of 180 K protein gene (940 nucleotides). Comparison of the sequence with homologous regions of thirteen other tobamovirus genomes showed that it had much higher identity to those of four other crucifer tobamoviruses, 85.2% to cr-TMV and turnip vein-clearing virus (TVCV), 87.4% to oilseed rape mosaic virus (ORMV) and 87.1% to TMV-Cg, than to those of other tobamoviruses. Thus CTMV-W was most similar to ORMV and TMV-Cg in sequence, but only marginally so, whereas the location and size of its MP gene was the same as cr-TMV amd TVCV. These results, together with other analyses, show that CTMV-W is a new crucifer tobamovirus, that the five crucifer tobamoviruses can be classified into two subgroups based on MP gene organization, and that the rate of sequence change is not the same in all lineages.
确定了一种对十字花科植物具有感染性的十字花科烟草花叶病毒芥末株（CTMV-W）的3'末端2378个核苷酸。这包括235个核苷酸的3'非编码区、外壳蛋白（CP）基因（468个核苷酸）、运动蛋白（MP）基因（798个核苷酸）以及180K蛋白基因的C末端部分通读片段（940个核苷酸）。将该序列与其他13种烟草花叶病毒基因组的同源区域进行比较，结果表明，与其他烟草花叶病毒相比，它与其他四种十字花科烟草花叶病毒的同源性更高，与cr-TMV和芜菁脉明病毒（TVCV）的同源性为85.2%，与油菜花叶病毒（ORMV）的同源性为87.4%，与TMV-Cg的同源性为87.1%。因此，CTMV-W在序列上与ORMV和TMV-Cg最为相似，但只是略微相似，而其MP基因的位置和大小与cr-TMV和TVCV相同。这些结果与其他分析一起表明，CTMV-W是一种新的十字花科烟草花叶病毒，这五种十字花科烟草花叶病毒可根据MP基因结构分为两个亚组，并且在所有谱系中序列变化率并不相同。
