Cai Li, Chen Kunrong, Zhang Xuejiang, Yan Liying, Hou Mingsheng, Xu Zeyong
Hubei Crop Disease Monitoring and Safety Control Key Laboratory, Department of Plant Science and Technology, Huazhong Agricultural University,Wuhan, China.
Biochem Genet. 2009 Aug;47(7-8):451-61. doi: 10.1007/s10528-009-9244-4. Epub 2009 May 17.
In China, the tobamovirus that infects oilseed rape has been misdiagnosed as Tobacco mosaic virus (TMV) based on its morphological similarity and serological relatedness. Recently, a tobamovirus has been isolated from oilseed rape in China, which we named Youcai mosaic virus Br (YoMV-Br), according to its biological and molecular characteristics. It had strong infectivity to Cruciferae but less to Solanaceae, Leguminosae, and Cucurbitaceae, and its virion morphology was consistent with that of the tobamoviruses. At high concentrations, it serologically cross reacted with TMV antiserum. The 3' terminal sequence (2,283 nucleotides) of YoMV-Br was determined, including the 3' noncoding region, the CP and MP genes, and the C-terminal part of the replicase gene. Between the MP and CP genes, 77 nucleotides overlapped. Compared with homologous regions of 21 recognized species of Tobamovirus, YoMV-Br had a much higher identity to crucifer species than to other tobamoviruses. Phylogenetic analysis demonstrated that YoMV-Br was closely related to the YoMV cluster of tobamoviruses and distantly to TMV, so that they likely belong to different strains of the same species.
在中国，由于形态相似性和血清学相关性，感染油菜的烟草花叶病毒属病毒一直被误诊为烟草花叶病毒（TMV）。最近，从中国油菜中分离出一种烟草花叶病毒属病毒，根据其生物学和分子特征，我们将其命名为油菜花叶病毒Br（YoMV-Br）。它对十字花科植物具有较强的感染力，而对茄科、豆科和葫芦科植物的感染力较弱，其病毒粒子形态与烟草花叶病毒属病毒一致。在高浓度下，它与TMV抗血清发生血清学交叉反应。测定了YoMV-Br的3'末端序列（2283个核苷酸），包括3'非编码区、CP和MP基因以及复制酶基因的C末端部分。MP和CP基因之间有77个核苷酸重叠。与21种已确认的烟草花叶病毒属病毒的同源区域相比，YoMV-Br与十字花科植物物种的同源性远高于其他烟草花叶病毒属病毒。系统发育分析表明，YoMV-Br与烟草花叶病毒属病毒的YoMV簇密切相关，与TMV关系较远，因此它们可能属于同一物种的不同株系。
