Ohkubo T, Tsukada A, Tanaka M, Nakashima K
Department of Biochemistry, Faculty of Medicine, Mie University, Japan.
Comp Biochem Physiol B Biochem Mol Biol. 1998 Jul;120(3):449-55. doi: 10.1016/s0305-0491(98)10029-9.
Pigeon growth hormone receptor (pGHR) cDNA has been cloned, sequenced, characterized and expressed in COS-7 cells. The predicted pGHR preprotein was composed of 611 amino acids and contained a putative signal peptide, a single transmembrane region and the conserved proline-rich box 1 domain in the cytoplasmic region. A canonical polyadenylation signal was found in the extracellular region, as was observed in chicken growth hormone receptor cDNA. Northern blot analysis identified a single species of pGHR mRNA and its expression was widely observed in tissues in the young male pigeon. A pGHR expression vector was constructed and transfected into COS-7 cells. The transfected cells were specifically bound by 125I-labeled chicken GH and the binding was suppressed by the addition of excess amounts of unlabeled chicken GH.
鸽生长激素受体（pGHR）cDNA已被克隆、测序、鉴定并在COS-7细胞中表达。预测的pGHR前体蛋白由611个氨基酸组成，包含一个假定的信号肽、一个单一跨膜区域以及细胞质区域中保守的富含脯氨酸的盒1结构域。在细胞外区域发现了一个典型的多聚腺苷酸化信号，这在鸡生长激素受体cDNA中也有观察到。Northern印迹分析鉴定出一种单一的pGHR mRNA，并且在年轻雄性鸽的组织中广泛观察到其表达。构建了一个pGHR表达载体并转染到COS-7细胞中。转染的细胞被125I标记的鸡生长激素特异性结合，并且通过添加过量的未标记鸡生长激素可抑制这种结合。
