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了解过氧化物酶的血红素腔结构：电子吸收光谱和共振拉曼光谱与晶体学结果的比较。

Understanding heme cavity structure of peroxidases: comparison of electronic absorption and resonance Raman spectra with crystallographic results.

作者信息

Smulevich G

机构信息

Dipartimento di Chimica, Universitá di Firenze, Italy.

出版信息

Biospectroscopy. 1998;4(5 Suppl):S3-17. doi: 10.1002/(SICI)1520-6343(1998)4:5+<S3::AID-BSPY2>3.0.CO;2-R.

DOI:10.1002/(SICI)1520-6343(1998)4:5+<S3::AID-BSPY2>3.0.CO;2-R
PMID:9787910
Abstract

Electronic absorption and resonance Raman spectra of various peroxidases and selected site-directed mutants are reported. These results and the X-ray crystal structure data are critically analyzed and underline the differences that exist between the crystal and solution states. The effect of the vinyl conjugation on the electronic absorption maxima and the influence of the ligand nature on the wavelength of the charge-transfer (CT1) band are shown to be useful probes of subtle interactions in the heme pocket. The spectroscopic differences observed between the three classes of peroxidases are discussed in terms of their structural diversity.

摘要

报道了各种过氧化物酶和选定的定点突变体的电子吸收光谱和共振拉曼光谱。对这些结果和X射线晶体结构数据进行了严格分析，并强调了晶体态和溶液态之间存在的差异。乙烯基共轭对电子吸收最大值的影响以及配体性质对电荷转移（CT1）带波长的影响被证明是血红素口袋中微妙相互作用的有用探针。根据三类过氧化物酶的结构多样性，讨论了观察到的光谱差异。

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