Junta C M, Passos G A
Departamento de Genética, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Brasil.
Mol Cell Biochem. 1998 Oct;187(1-2):67-72. doi: 10.1023/a:1006807021251.
The maturation of T-cells depends on V(D)J recombination at the TCR alpha/beta and gamma/delta loci that occurs in the thymus during fetal development. Due to the essential role of the TCRbeta in thymocyte differentiation, it is important to define with precision the temporal emergence of the TCRbeta rearrangement and its expression in normal non-manipulated mouse strains. We studied the onset of the V(D)J recombination and transcription of the TCR Valpha3 and Vbeta11 genes during ontogeny in Balb-c, C57B1/6 and CBA inbred mouse strains. Our data show differences in the emergence of recombination in both TCR alpha and beta loci among strains. The transcriptions of these loci followed respective recombinations and we detected an early germline transcript before TCR beta locus recombination in the CBA strain.
T细胞的成熟取决于胎儿发育期间在胸腺中发生的TCRα/β和γ/δ基因座处的V(D)J重组。由于TCRβ在胸腺细胞分化中起着至关重要的作用，因此精确确定TCRβ重排的时间出现及其在正常未处理小鼠品系中的表达非常重要。我们研究了Balb-c、C57B1/6和CBA近交系小鼠个体发育过程中TCR Vα3和Vβ11基因的V(D)J重组和转录的起始情况。我们的数据显示，不同品系的TCRα和β基因座在重组出现方面存在差异。这些基因座的转录遵循各自的重组情况，并且我们在CBA品系中检测到TCRβ基因座重组之前的早期种系转录本。
