Nomiyama T, Doi Y, Kudo H, Furukawa H, Hamasaki K, Fujimoto S
Department of Anatomy, University of Occupational and Environmental Health, School of Medicine, Kitakyushu, Japan.
Acta Anat (Basel). 1998;162(1):23-32. doi: 10.1159/000046465.
The differentiating cavernous body (CB) of postnatal rabbit penises was examined with a special reference to immunolocalizations for fibronectin (FN) and endothelin-1 (ET-1). At postnatal day 1, the CBs were embedded by an abundance of mesenchymal cells (MCs), and some of them were closely associated with endothelial cells of preexisting capillaries. Our electron micrographs indicated that such MCs are successively incorporated into the capillary endothelium as vasoformative cells. At this period, vascular sprouts of the helicine artery (HA), which were associated with the MCs, arose from the deep penile artery, and the transformation of such cells to endothelial and medial muscle ones was also indicated, and some MCs appeared to differentiate to epithelioid cells in the media. Immunoreactions for FN were preferentially localized in the rough endoplasmic reticulum (rER) and along the plasma membrane of such vasoformative MCs, and on the extracellular matrix components which connect these MCs with sprouts of both growing capillaries and HA. These findings suggest that FN, which is produced in the rER of the MCs, plays a crucial role in the mechanical linkage during the incorporation of vasoformative MCs into these penile vessels. Immunoreactions for ET-1 were preferentially localized on Weibel-Palade bodies in endothelial cells of the HA, implying the involvement of this peptide in the regulation of the local blood flow in this vessel.
对出生后兔阴茎的海绵体（CB）进行了鉴别研究，特别关注纤连蛋白（FN）和内皮素-1（ET-1）的免疫定位。出生后第1天，海绵体被大量间充质细胞（MCs）包绕，其中一些与已存在的毛细血管内皮细胞紧密相连。我们的电子显微镜图像显示，这些间充质细胞作为血管形成细胞逐渐整合到毛细血管内皮中。在此期间，与间充质细胞相关的螺旋动脉（HA）的血管芽从阴茎深部动脉发出，同时也显示出这些细胞向内皮细胞和平滑肌细胞的转化，一些间充质细胞似乎在中膜分化为类上皮细胞。FN的免疫反应优先定位于这些血管形成间充质细胞的粗面内质网（rER）和沿质膜，以及将这些间充质细胞与生长中的毛细血管和HA的芽连接起来的细胞外基质成分上。这些发现表明，在间充质细胞整合到这些阴茎血管的过程中，由间充质细胞的rER产生的FN在机械连接中起关键作用。ET-1的免疫反应优先定位于HA内皮细胞的Weibel-Palade小体上，这意味着该肽参与了该血管局部血流的调节。
