Thompson P M, Rosenberger C, Holt S, Perrone-Bizzozero N I
Department of Psychiatry, University of New Mexico School of Medicine, USA.
J Psychiatr Res. 1998 Sep-Oct;32(5):297-300. doi: 10.1016/S0022-3956(98)00018-1.
Changes in the quantity and distribution of neuronal proteins have been reported in psychiatric and neurological illnesses. The majority of this work has been performed in post-mortem samples and the results are difficult to apply to clinical care. The objective of this study is to develop a methodology that can identify trace amounts of brain proteins in cerebral spinal fluid (CSF). Human cerebral spinal fluid was processed to remove albumin and immunoglobulins. CSF samples were analyzed on Western blots using a monoclonal antibody against SNAP-25. These samples were compared to SNAP-25 immunoprecipitated from CSF, rat and human brain homogenates. The monoclonal antibody Mab 331 identified a single band of 25 kDa in all samples. These results demonstrate that the presynaptic protein SNAP-25 can be identified and measured in CSF.
在精神疾病和神经疾病中，已报道了神经元蛋白的数量和分布变化。这项工作大多是在尸检样本中进行的，其结果难以应用于临床护理。本研究的目的是开发一种能够识别脑脊液（CSF）中痕量脑蛋白的方法。对人类脑脊液进行处理以去除白蛋白和免疫球蛋白。使用抗SNAP - 25单克隆抗体在蛋白质印迹法上分析脑脊液样本。将这些样本与从脑脊液、大鼠和人类脑匀浆中免疫沉淀的SNAP - 25进行比较。单克隆抗体Mab 331在所有样本中均鉴定出一条25 kDa的单条带。这些结果表明，突触前蛋白SNAP - 25可在脑脊液中被识别和测量。
