Eppihimer M J, Russell J, Langley R, Vallien G, Anderson D C, Granger D N
Department of Molecular and Cellular Physiology, Louisiana State University Medical Center, Shreveport 71130-3932, USA.
Microcirculation. 1998;5(2-3):179-88.
To characterize and compare the expression of PECAM-1 in unstimulated and tumor necrosis factor alpha-(TNF-alpha)-stimulated tissues of mice.
Binding and non-binding monoclonal antibodies (mAb) were radiolabeled and injected into wild-type mice and mice deficient of P-selectin, CD18, or ICAM-1. The relative accumulation of binding mAb (PECAM-1 mAb) was determined in wild-type mice following a 25 micrograms/kg i.p. injection of TNF-alpha and in mutant mice under basal conditions.
Under unstimulated conditions, PECAM-1 was significantly expressed in all tissues examined, with no changes occurring after TNF-alpha stimulation. An equivalence of PECAM-1 expression was observed in unstimulated tissues among wild-type mice and mice that are genetically deficient in either CD18, ICAM-1, or P-selectin. The level of PECAM-1 expression in different vascular beds was highly correlated to published values of endothelial surface area. Normalization of previously published values of ICAM-1, VCAM-1, E- and P-selectin expression relative to PECAM-1 expression in the same tissues revealed a diminished organ-to-organ variability in expression of the different adhesion molecules. Estimates of adhesion molecule expression in lung and brain were most profoundly affected by normalization to PECAM-1 expression.
These findings support the use of PECAM-1 expression in regional vascular beds as an indicator of endothelial cell surface area.
对小鼠未受刺激及经肿瘤坏死因子α（TNF-α）刺激的组织中PECAM-1的表达进行表征和比较。
将结合型和非结合型单克隆抗体（mAb）进行放射性标记，然后注射到野生型小鼠以及缺乏P-选择素、CD18或ICAM-1的小鼠体内。在野生型小鼠腹腔注射25微克/千克TNF-α后以及在基础条件下的突变小鼠中，测定结合型mAb（PECAM-1 mAb）的相对蓄积量。
在未受刺激的条件下，PECAM-1在所有检测的组织中均有显著表达，TNF-α刺激后无变化。在野生型小鼠以及基因上缺乏CD18、ICAM-1或P-选择素的小鼠的未受刺激组织中，观察到PECAM-1表达相当。不同血管床中PECAM-1的表达水平与已发表的内皮表面积值高度相关。将同一组织中ICAM-1、VCAM-1、E-选择素和P-选择素表达的先前发表值相对于PECAM-1表达进行归一化处理后，发现不同黏附分子表达的器官间变异性减小。肺和脑中黏附分子表达的估计值受归一化至PECAM-1表达的影响最为显著。
这些发现支持将区域血管床中PECAM-1的表达用作内皮细胞表面积的指标。
