Gamrin L, Berg H E, Essén P, Tesch P A, Hultman E, Garlick P J, McNurlan M A, Wernerman J
Department of Anaesthesiology and Intensive Care, Huddinge University Hospital, Sweden.
Acta Physiol Scand. 1998 Aug;163(4):369-77. doi: 10.1046/j.1365-201X.1998.t01-1-00391.x.
Atrophy of skeletal muscle is observed in response to immobilization and lack of weight-bearing. The aim of this study was to investigate the effect of immobilization on muscle protein synthesis and associated biochemical parameters in skeletal muscle of healthy volunteers employing a standardized model of lower limb unloading. One leg was unloaded for 10 days, and percutaneous muscle biopsies were taken before and at the end of the unloading period. The capacity for protein synthesis, as reflected by the concentration of RNA, decreased by 16% (P < 0.05) although the fractional synthesis rate (FSR) of protein was not significantly changed after 10 days of unloading. Furthermore there was an increase in the concentration of the free branched chain amino acids in muscle by 48% (P < 0.05).
在因固定不动和缺乏负重而导致的情况下，可观察到骨骼肌萎缩。本研究的目的是采用标准化的下肢卸载模型，调查固定不动对健康志愿者骨骼肌中肌肉蛋白质合成及相关生化参数的影响。一条腿卸载10天，在卸载期开始前和结束时进行经皮肌肉活检。尽管卸载10天后蛋白质的分数合成率（FSR）没有显著变化，但RNA浓度所反映的蛋白质合成能力下降了16%（P<0.05）。此外，肌肉中游离支链氨基酸的浓度增加了48%（P<0.05）。
