Rodríguez R R, Renauld A, Celener D, Pérez R L, Susemihl M C
Department of Physiology, Medical School, Buenos Aires University, Paraguay, Argentina.
Diabetes Res Clin Pract. 1998 Aug;41(2):95-100. doi: 10.1016/s0168-8227(98)00073-4.
It has been demonstrated that, in the diabetic rat, pregnancy and lactation are severely altered: in this study, we have measured the size of Langerhans islets of rat pups, the offspring of experimental diabetic mothers and nondiabetic controls. Diabetes was induced through streptozotocin administration (dose, 60 mg/kg body wt.). This drug was injected in every animal; their blood sugar was measured 1 week later (Haemo-Glukotest, Boehringer Mannheim), and they were then separated into three groups according to their fasting blood sugar levels: (a) severe diabetics (above 16.5 mM/l); (b) mild diabetics (6.5-16.5 mM/l); and (c) nondiabetic normals. They received insulin therapy (2-4 I.U./day) as the mild diabetics exhibited a slightly higher than normal fasting blood sugar, and the diabetic ones, above 15 mM/l. The areas of Langerhans islets of pups were measured 1 and 5 days after parturition; pancreas sections were dyed (haematoxylin-eosin) and morphometry was then performed using a digitalized magnetic tabloid connected to a Zeiss Morphomat 30 (Kontron). On the first day after parturition, the pancreas section areas in pups from mildly and severely diabetic mothers were smaller than those in neonates from nondiabetic controls (P < 0.001). The areas in neonates from severely diabetic mothers showed a more intense decrease than those from mildly diabetic animals (P < 0.01). On day 5 after delivery, the areas of Langerhans islets in offspring from normal mothers decreased and those in pups from diabetic mothers tended to normalize (P < 0.01), particularly those from the severely sick group (P < 0.01). We conclude that after parturition the offspring is no longer exposed to the high blood sugar levels found in both diabetic groups of mothers, thereby no hyperinsulinemia is needed; as time elapses, then, the area of their Langerhans islets tends to normalization.
已证实，在糖尿病大鼠中，妊娠和哺乳会严重改变：在本研究中，我们测量了实验性糖尿病母亲和非糖尿病对照的后代大鼠幼崽的胰岛大小。通过注射链脲佐菌素（剂量为60mg/kg体重）诱导糖尿病。给每只动物注射这种药物；1周后测量它们的血糖（使用勃林格殷格翰的血糖仪），然后根据空腹血糖水平将它们分为三组：（a）重度糖尿病组（血糖高于16.5mM/l）；（b）轻度糖尿病组（血糖为6.5 - 16.5mM/l）；（c）非糖尿病正常组。由于轻度糖尿病组的空腹血糖略高于正常水平，糖尿病组的空腹血糖高于15mM/l，所以对它们进行胰岛素治疗（每天2 - 4国际单位）。在分娩后1天和5天测量幼崽胰岛的面积；将胰腺切片染色（苏木精 - 伊红染色），然后使用连接到蔡司Morphomat 30（康创公司）的数字化磁性载玻片进行形态测量。在分娩后的第一天，轻度和重度糖尿病母亲的幼崽的胰腺切片面积小于非糖尿病对照的新生儿（P < 0.001）。重度糖尿病母亲的新生儿的胰腺切片面积下降比轻度糖尿病动物的新生儿更明显（P < 0.01）。在分娩后第5天，正常母亲的后代的胰岛面积减小，糖尿病母亲的幼崽的胰岛面积趋于正常（P < 0.01），特别是重病组的幼崽（P < 0.01）。我们得出结论，分娩后，后代不再暴露于两组糖尿病母亲中发现的高血糖水平，因此不需要高胰岛素血症；随着时间的推移，它们的胰岛面积趋于正常。
