在过表达人乙酰胆碱酯酶变体的转基因小鼠中的位置效应斑驳和脑特异性沉默。

Position effect variegations and brain-specific silencing in transgenic mice overexpressing human acetylcholinesterase variants.

作者信息

Sternfeld M, Patrick J D, Soreq H

机构信息

Department of Biological Chemistry, Hebrew University of Jerusalem, Israel.

出版信息

J Physiol Paris. 1998 Jun-Aug;92(3-4):249-55. doi: 10.1016/s0928-4257(98)80028-4.

DOI:10.1016/s0928-4257(98)80028-4

PMID:9789818

Abstract

Position effect variegations as well as brain-specific silencing were observed in novel transgenic mouse pedigrees expressing human acetylcholinesterase (AChE) variants. Muscle AChE activities varied between 1.6- and 350-fold of control in these lines, one carrying insertion-inactivated InE6-AChE and two with 'readthrough' I4/E5 AChE, all under control of the ubiquitous CMV promoter. In contrast, brain AChE levels remained within a range of 1.5-fold over control, suggesting an upper limit of brain AChE which is compatible with life.

摘要

在表达人乙酰胆碱酯酶（AChE）变体的新型转基因小鼠谱系中观察到位置效应斑驳以及脑特异性沉默。在这些品系中，肌肉AChE活性在对照的1.6至350倍之间变化，一个携带插入失活的InE6-AChE，两个携带“通读”I4/E5 AChE，所有这些都在普遍存在的CMV启动子的控制下。相比之下，脑AChE水平保持在对照的1.5倍范围内，表明脑AChE存在与生命相容的上限。