Obata F, Tsunoda-Iizuka M
Department of Immunology, Kitasato University School of Allied Health Sciences, Sagamihara, Japan.
Scand J Immunol. 1998 Oct;48(4):364-70. doi: 10.1046/j.1365-3083.1998.00408.x.
We examined whether individuals with an identical HLA-DR type utilized the same T-cell receptors (TCRs) to recognize a given allogeneic HLA-DR molecule. CD4+ T cells from three responder-cell donors possessing the DRB0901 allele were stimulated with HLA-DRB10406 molecules, subjected to the primary mixed lymphocyte reaction (MLR) and the TCRs of the activated CD4+ T cells were analysed using single strand conformation polymorphism (SSCP) and random cDNA clone sequencing. The responder cells of each donor yielded many dominant SSCP bands in several TCRAV and TCRBV segments, but none of these dominant SSCP bands derived from two or three responders. Random cDNA sequence analysis demonstrated that the alloreactive TCRs were diverse, but each of the three responder-cell donors showed some dominant cDNA clones. However, no amino acid sequence identities or similarities among the dominant cDNAs of these donors were detected. These results indicate that certain T-cell clones from each individual's TCR repertoire pool expand preferentially as a result of allogeneic HLA-DR recognition but these clones are not necessarily common to different individuals, even when their responder cells possess identical DR alleles and are stimulated with the same alloantigen.
我们研究了具有相同HLA - DR类型的个体是否利用相同的T细胞受体（TCR）来识别给定的同种异体HLA - DR分子。用HLA - DRB10406分子刺激来自三名具有DRB0901等位基因的反应细胞供体的CD4 + T细胞，使其经历初次混合淋巴细胞反应（MLR），并使用单链构象多态性（SSCP）和随机cDNA克隆测序分析活化的CD4 + T细胞的TCR。每个供体的反应细胞在几个TCRAV和TCRBV区段产生了许多显性SSCP条带，但这些显性SSCP条带中没有一个来自两个或三个反应者。随机cDNA序列分析表明，同种异体反应性TCR是多样的，但三个反应细胞供体中的每一个都显示出一些显性cDNA克隆。然而，在这些供体的显性cDNA之间未检测到氨基酸序列同一性或相似性。这些结果表明，由于同种异体HLA - DR识别，来自每个个体TCR库的某些T细胞克隆优先扩增，但这些克隆不一定是不同个体共有的，即使它们的反应细胞具有相同的DR等位基因并受到相同的同种异体抗原刺激。
