Kariuki M M, Kiaira J K, Mulaa F K, Mwangi J K, Wasunna M K, Martin S K
Department of Biochemistry, University of Nairobi, Kenya.
Am J Trop Med Hyg. 1998 Oct;59(4):505-8. doi: 10.4269/ajtmh.1998.59.505.
Cultivated Plasmodium falciparum gametocytes reach maturity in vitro in approximately 14-16 days, during which they pass through five morphologically distinct developmental stages. Purification of the earlier developmental stages has not been previously reported. We have modified the standard discontinuous Percoll gradient method for the separation of stage IV and V gametocytes to obtain enriched preparations of those and the earlier P. falciparum gametocyte stages. In contrast to the stages II, III, and IV, the mature stage V gametocytes from our gradient readily transformed into gametes. Such preparations may be useful in research studies on the mechanisms that underlie gametocytogenesis.
体外培养的恶性疟原虫配子体大约在14 - 16天内成熟，在此期间它们会经历五个形态上不同的发育阶段。此前尚未有关于早期发育阶段纯化的报道。我们改进了用于分离IV期和V期配子体的标准不连续 Percoll 梯度法，以获得这些阶段以及早期恶性疟原虫配子体阶段的富集制剂。与II期、III期和IV期不同，我们梯度分离得到的成熟V期配子体很容易转化为配子。这样的制剂可能有助于对配子体发生机制的研究。
