Kawakami K, Yasuda J, Shiraishi M, Kayama T, Doi K, Perucho M, Sekiya T
Oncogene Division, National Cancer Center Research Institute, 1-1, Tsukiji 5-Chomoe, Chuo-ku, Tokyo, 104-0045, Japan.
Biochem Biophys Res Commun. 1998 Oct 9;251(1):153-7. doi: 10.1006/bbrc.1998.9418.
DNA fingerprinting using arbitrarily primed PCR (AP-PCR) is useful for detecting cancer-specific DNA aberrations without targeting any particular genes or knowing any nucleotide sequences in advance. AP-PCR fingerprinting is an efficient method for finding loss of anonymous chromosomal regions in cancers. We analyzed DNA from 44 human non-small cell lung cancers by fingerprinting using a single primer and found a loss of signal intensity in a DNA fragment amplified from chromosome 10 (fragment F) in 15 tumors. The detailed location of the fragment F locus on chromosome 10q was determined by PCR-based analysis of radiation hybrid panels using a sequence-tagged site established for the fragment. In 12 of the 15 tumors, loss of the signal detected by AP-PCR fingerprinting was in agreement with the results obtained by analysis of allelic imbalances using 7 polymorphic CA-microsatellite DNA markers for loci around the fragment F locus (p=0.0009). We conclude that a hitherto unknown suppressor gene for lung cancer resides at 10q in the vicinity of fragment F.
使用任意引物PCR（AP-PCR）进行DNA指纹分析，有助于在不预先针对任何特定基因或知晓任何核苷酸序列的情况下，检测癌症特异性DNA畸变。AP-PCR指纹分析是一种在癌症中发现无名染色体区域缺失的有效方法。我们使用单一引物通过指纹分析对44例人类非小细胞肺癌的DNA进行了分析，发现在15个肿瘤中，从10号染色体扩增的一个DNA片段（片段F）的信号强度有所缺失。通过使用为该片段建立的序列标签位点，基于辐射杂种细胞板的PCR分析确定了片段F在10q染色体上的详细位置。在15个肿瘤中的12个中，AP-PCR指纹分析检测到的信号缺失与使用7个多态性CA微卫星DNA标记对片段F位点周围位点进行等位基因不平衡分析的结果一致（p = 0.0009）。我们得出结论，一个迄今未知的肺癌抑制基因位于10q上片段F附近。
