Zhang Z, Chen J, Jin D
Nan Fang Hospital, First Military Medical University, Guangzhou, (510515), People's Republic of China.
Biochem Biophys Res Commun. 1998 Oct 9;251(1):190-4. doi: 10.1006/bbrc.1998.9412.
The growth-promoting activity of PDGF-BB was studied on the adult osteoclasts in the present study. The PDGF receptor beta was detected on the osteoclast membrane through immunohistochemistry (LSAB method) and immunomicroscopy. The PDGF-BB was exerted on the osteoclasts that adhered to the bone slice at concentrations of 0, 10, 20, 30, and 40 ng/ml. The volume of Howship's lacuna augmented significantly and the number of resorption pits also increased with its dose (p < 0.01). The activity of both total acid phosphatase (ACP) and tartrate-resistant acid phosphatase (TRAP) increased significantly. These results suggest that PDGF-BB promotes adult osteoclastic bone resorption directly through PDGF receptor beta and is believed to play important roles in the bone healing process and reconstruction.
本研究对血小板源性生长因子BB（PDGF - BB）在成年破骨细胞上的促生长活性进行了研究。通过免疫组织化学（LSAB法）和免疫显微镜技术在破骨细胞膜上检测到了血小板源性生长因子受体β。将浓度为0、10、20、30和40 ng/ml的PDGF - BB作用于黏附在骨切片上的破骨细胞。随着PDGF - BB剂量增加，豪希普陷窝的体积显著增大，吸收小凹的数量也增加（p < 0.01）。总酸性磷酸酶（ACP）和抗酒石酸酸性磷酸酶（TRAP）的活性均显著增加。这些结果表明，PDGF - BB通过血小板源性生长因子受体β直接促进成年破骨细胞的骨吸收，并且被认为在骨愈合过程和重建中发挥重要作用。
