Girón M D, Vargas A M, Suárez M D, Salto R
Facultad de Farmacia, Universidad de Granada, Campus de Cartuja s/n, Granada, 18071, Spain.
Biochem Biophys Res Commun. 1998 Oct 9;251(1):230-4. doi: 10.1006/bbrc.1998.9446.
The receptor for advanced glycosylation end products (RAGE) is an integral membrane protein responsible for the recognition and internalization of those extensively modified proteins. The receptor has an extracellular domain that binds to the advanced glycosylation end products. By reverse-transcription and polymerase chain reaction amplification, we have identified in rat liver and kidney two amplified products that correspond to cDNA coding for a part of the extracellular domain of the receptor. Sequencing of these products showed that these amplified molecules were similar except for a 27-bp fragment that was absent in the smaller product. This spliced region is located close to the transmembrane region of the receptor. We have confirmed the possibility of the alternative splicing in the generation of these mRNA isoforms by cloning a fragment of the rat gene for RAGE. This fragment has a distribution of introns and exons fully compatible with the proposed alternative splicing.
晚期糖基化终末产物受体（RAGE）是一种整合膜蛋白，负责识别和内化那些高度修饰的蛋白质。该受体具有一个与晚期糖基化终末产物结合的细胞外结构域。通过逆转录和聚合酶链反应扩增，我们在大鼠肝脏和肾脏中鉴定出两个扩增产物，它们对应于编码该受体细胞外结构域一部分的cDNA。这些产物的测序表明，除了较小产物中缺失的一个27bp片段外，这些扩增分子相似。这个剪接区域靠近受体的跨膜区域。通过克隆大鼠RAGE基因的一个片段，我们证实了在这些mRNA异构体产生过程中存在可变剪接的可能性。这个片段的内含子和外显子分布与所提出的可变剪接完全相符。
