Revill P A, Davidson A D, Wright P J
Department of Microbiology, Monash University, Wellington Rd., Clayton, Victoria, 3168, Australia.
Virology. 1998 Sep 30;249(2):231-7. doi: 10.1006/viro.1998.9345.
Mushroom bacilliform virus (MBV) is often found in cultivated mushrooms (Agaricus bisporus) with La France disease. MBV has a 4-kb ssRNA genome of positive-sense encoding four major open reading frames (ORFs). The arrangement of ORFs at the 5' end of the genome and the deduced amino-acid sequences of two of the putative gene products (protease and RNA-dependent RNA polymerase) show remarkable similarity to some plant viruses, particularly subgroup II luteoviruses. We show that this similarity extends to the translation strategy at the 5' end of the genome, the presence of a genome-linked protein (VPg), and the location of the VPg downstream of the protease motifs in the polypeptide encoded by ORF2.
蘑菇杆状病毒（MBV）常出现在患有拉弗朗斯病的栽培蘑菇（双孢蘑菇）中。MBV具有一个4kb的正义单链RNA基因组，编码四个主要的开放阅读框（ORF）。基因组5'端ORF的排列以及两个推定基因产物（蛋白酶和RNA依赖性RNA聚合酶）的推导氨基酸序列与一些植物病毒，特别是II亚组黄症病毒，显示出显著的相似性。我们表明，这种相似性延伸到基因组5'端的翻译策略、基因组连接蛋白（VPg）的存在以及VPg在ORF2编码的多肽中蛋白酶基序下游的位置。
