Hemmer S, Wasenius V M, Knuutila S, Joensuu H, Franssila K
Department of Oncology, Helsinki University Central Hospital, Finland.
Br J Cancer. 1998 Oct;78(8):1012-7. doi: 10.1038/bjc.1998.620.
DNA copy number changes were compared in 29 histologically benign follicular adenomas, of which five were atypical, and 13 follicular carcinomas of the thyroid by comparative genomic hybridization. DNA copy number changes were frequent in adenomas (14 out of 29, 48%). Most changes were gains, and they always involved a gain of the entire chromosome 7 (10 out of 29, 34%); other common gains involved chromosomes 5 (28%), 9 (10%), 12 (24%), 14 (21%), 17 (17%), 18 (14%) and X (17%). Losses were found only in four (14%) adenomas. Two of the five atypical adenomas had DNA copy number losses, and none had gains. Unlike adenomas, gains were rare and losses were frequent in carcinomas. A loss of chromosome 22 or 22q was particularly common in carcinomas (6 out of 13, 46%), whereas a loss of chromosome 22 was found in only two (7%) adenomas, one of which was atypical (P = 0.002). A loss of 1p was also frequent in carcinomas (31%), but gains of chromosomes 5, 7, 12, 14 or X that were common in adenomas were not found. Loss of chromosome 22 or 22q was present in six of the eight widely invasive follicular carcinomas, but in only one of the five minimally invasive carcinomas. We conclude that large DNA copy number changes are common in thyroid adenomas. These changes are strikingly different from those found in follicular carcinomas consisting of few losses and frequent gains, especially those of chromosome 7. A loss of chromosome 22 is common in widely invasive follicular carcinoma.
通过比较基因组杂交技术，对29例组织学诊断为良性的滤泡性腺瘤（其中5例为非典型性腺瘤）和13例甲状腺滤泡癌的DNA拷贝数变化进行了比较。腺瘤中DNA拷贝数变化较为常见（29例中有14例，占48%）。大多数变化为扩增，且总是涉及整个7号染色体的扩增（29例中有10例，占34%）；其他常见的扩增涉及5号染色体（28%）、9号染色体（10%）、12号染色体（24%）、14号染色体（21%）、17号染色体（17%）、18号染色体（14%）和X染色体（17%）。仅在4例（14%）腺瘤中发现了缺失。5例非典型腺瘤中有2例存在DNA拷贝数缺失，无一例有扩增。与腺瘤不同，癌中扩增少见而缺失常见。22号染色体或22q的缺失在癌中尤为常见（13例中有6例，占46%），而仅在2例（7%）腺瘤中发现22号染色体缺失，其中1例为非典型腺瘤（P = 0.002）。1p的缺失在癌中也很常见（31%），但未发现腺瘤中常见的5号、7号、12号、14号或X染色体的扩增。8例广泛浸润性滤泡癌中有6例存在22号染色体或22q缺失，但5例微浸润性癌中仅有1例存在该缺失。我们得出结论，甲状腺腺瘤中DNA拷贝数的大变化很常见。这些变化与滤泡癌中发现的变化显著不同，滤泡癌中缺失较少而扩增频繁，尤其是7号染色体的扩增。22号染色体缺失在广泛浸润性滤泡癌中很常见。
