Sawaya R, Go Y, Kyritisis A P, Uhm J, Venkaiah B, Mohanam S, Gokaslan Z L, Rao J S
Department of Neurosurgery, Department of Neuro-Oncology, The University of Texas M. D. Anderson Cancer Center, 1515 Holcombe Boulevard, Houston, Texas, 77030, USA.
Biochem Biophys Res Commun. 1998 Oct 20;251(2):632-6. doi: 10.1006/bbrc.1998.9466.
Our previous studies demonstrated that matrix metalloproteinase (MMP-9) levels were significantly higher in human glioblastoma tissue samples than in low-grade brain tumors and normal brain tissue (Rao et al., Cancer Res. 53, 2208-2211, 1993). In the present study, we measured the levels of MMP-2 and MMP-9 during the growth of glial tumors in nude mice by intracerebral injection of glioblastoma cells. Using gelatin zymography, densitometry, and an enzyme-linked immunosorbent assay, we found that the enzyme activity and protein count of MMP-2 and MMP-9 were a respective 3- to 10- and 2- to 30-fold higher in tumors at day 14 and 28 than in normal tissue. Immunohistochemical staining for MMP-9 showed strong immunoreactivity in tumor cells and the staining intensity was much higher at day 28, compared to day 14. These results suggest that upregulation of MMP-9 plays a major role in the glioma tumor growth in vivo.
我们之前的研究表明，人胶质母细胞瘤组织样本中的基质金属蛋白酶（MMP - 9）水平显著高于低级别脑肿瘤和正常脑组织（Rao等人，《癌症研究》53卷，2208 - 2211页，1993年）。在本研究中，我们通过向裸鼠脑内注射胶质母细胞瘤细胞，测量了胶质肿瘤生长过程中MMP - 2和MMP - 9的水平。使用明胶酶谱法、光密度测定法和酶联免疫吸附测定法，我们发现，在第14天和第28天时，肿瘤中MMP - 2和MMP - 9的酶活性和蛋白含量分别比正常组织高3至10倍和2至30倍。MMP - 9的免疫组织化学染色显示肿瘤细胞中有强烈的免疫反应性，与第14天相比，第28天的染色强度要高得多。这些结果表明，MMP - 9的上调在体内胶质瘤肿瘤生长中起主要作用。
