Shitoh K, Konishi F, Masubuchi S, Senba S, Tsukamoto T, Kanazawa K
Department of Surgery, Jichi Medical School, Tochigi, Japan.
Jpn J Clin Oncol. 1998 Sep;28(9):538-41. doi: 10.1093/jjco/28.9.538.
DNA replication errors (RER) have been found in hereditary nonpolyposis colorectal carcinomas and in sporadic colorectal carcinomas. The incidence of RER depends on which and how many markers are examined. The main purpose of the present study was to determine the key markers for detecting RER most efficiently.
The RER status of 76 sporadic advanced colorectal carcinomas in the proximal colon were investigated. Seven microsatellite markers (D2S123, D3S1029, D3S1611, D2S72, TP53, Mfd26 and BAT26) were chosen to determine the RER status by PCR using the non-Rl method, because these seven markers have frequently been used in other studies and also detect RER.
It was found that 44.7% of sporadic colorectal advanced carcinomas in the proximal colon (34 of 76) showed RER at one or more loci. Among these 34 cases, RER was present at three or more markers (severe RER) in 22. All 22 of these cases showed RER at BAT26 and TP53. The other 12 cases with RER showed RER at one or two markers (mild RER). Eleven of these 12 cases (91%) showed RER at Mfd26 and there were one or two cases with mild RER at each of the other loci.
When one intends to analyze routinely a large number of cases, an analysis of two or three markers (Mfd26 and BAT26 or TP53) is considered to be sufficient for detecting mild and severe RER.
在遗传性非息肉病性结直肠癌和散发性结直肠癌中均发现了DNA复制错误（RER）。RER的发生率取决于所检测的标记物种类及数量。本研究的主要目的是确定最有效地检测RER的关键标记物。
对76例近端结肠散发性晚期结直肠癌的RER状态进行了研究。选择了7个微卫星标记物（D2S123、D3S1029、D3S1611、D2S72、TP53、Mfd26和BAT26），采用非Rl法通过PCR来确定RER状态，因为这7个标记物在其他研究中经常被使用且也能检测RER。
发现近端结肠散发性晚期结直肠癌中有44.7%（76例中的34例）在一个或多个位点显示RER。在这34例中，22例（22/34）在三个或更多标记物处存在RER（严重RER）。所有这22例在BAT26和TP53处均显示RER。另外12例有RER的病例在一个或两个标记物处显示RER（轻度RER）。这12例中的11例（91%）在Mfd26处显示RER，在其他位点各有1或2例轻度RER。
当打算常规分析大量病例时，分析两个或三个标记物（Mfd26和BAT26或TP53）被认为足以检测轻度和重度RER。
